| 研究実績の概要 |
In our research, we demonstrated the generation of human CDX2+ posterior gut endoderm cells (PGECs) from induced pluripotent stem cell (iPSC) by manipulating FGF, TGF and Wnt signaling pathways. By screening the gut elongation factors, combinatorial EGF, VEGF, FGF2, Chir99021 and A83-01 treatments amplify storable PGECs over a number of passages up to 1021 cells. PGECs, showed more stable differentiation propensity into liver, pancreas and gut lineage cells both in vitro and in vivo. Human PGECs liver bud organoids, showed therapeutic potential against fulminant liver failure upon transplantation.
Together, the use of PGECs, as an alternative to PSC, may be a stable cellular source of endoderm-derived organoids for studying human development, modeling disease and ultimately therapy.
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