| 研究実績の概要 |
The original proposal was to identify the potential role of RNA decay in mediating changes of the eventual RNA abundance.
In the last fiscal year, I have refined the RNA decay profile of 5-bromouridine immunoprecipitation pulse chase sequencing (BRIC-seq), where I have used thresholds to filter genes to ensure that I compare RNA decay for genes with RNA half-life that can be precisely determined.
I have managed to identify the set of genes where the changes in RNA decay does not result in changes in the eventual RNA abundance, suggesting some form of transcriptional feedback to maintain the level of RNA abundance. Additionally, I have overlaid the ENCODE transcription factor ChIP-seq based binding site data, and I have found several transcription factors that enrich in the promoter regions of these genes, which could explain the feedback mechanisms.
In addition, through the comparison of HIF-1 ChIP-seq data to the RNA-seq and BRIC-seq (RNA decay) data, I have preliminarily found that RNA abundance regulation through HIF-1 and RNA decay are distinct.
Altogether, in this year I have managed to gather enough material to summarise the findings into a journal publication. To have the research published, I will conduct further validations on the transcription factors and RNA decay factors that mediate changes in RNA abundance.
|
