| 研究課題/領域番号 |
15K06729
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| 研究機関 | 公益財団法人東京都医学総合研究所 |
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研究代表者 |
堀内 純二郎 公益財団法人東京都医学総合研究所, 認知症・高次脳機能研究分野, 主席研究員 (80392364)
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| 研究期間 (年度) |
2015-04-01 – 2018-03-31
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| キーワード | 加齢性記憶障害 / グリア細胞 / ショウジョウバエ / 長期記憶 |
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| 研究実績の概要 |
We are studying the causes of age-related memory impairment (AMI) using Drosophila as a model organism. Previously, we showed that aging affects two types of memory in flies, a short lasting form that can be measured 1 hr after a single training session, and protein synthesis-dependent long-term memory, which can be measured 24 hrs after multiple training sessions. We found that both of these memory defects are caused by age-related dysfunction of glial activity, and are currently identifying the mechanisms through which this occurs.
Defects in 1 hr memory are caused by an age-dependent decrease in production of a neuromodulator, D-serine. Memory and other brain functions require neuronal signaling, which, in turn, requires activity of neurotransmitters and neuromodulators. The neuromodulator, D-serine, is produced by glial cells, and is required for NMDA-type glutamate receptor signaling. We have found that D-serine amounts decrease upon aging, and artificially increasing amounts of D-serine prevents age-associated reductions in 1 hr memory. We show that old glial cells produce increased amounts of a protein, pyruvate carboxylase, which inhibits D-serine production. Thus, we find that we can improve 1 hr memory in old flies by either inhibiting pyruvate carboxylase activity or increasing D-serine amounts.
We are currently elucidating mechanisms causing decreased long-term memory in old flies.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
To determine why aging causes decreases in long-term memory (LTM), we first examined the function of glia in LTM formation in young flies. We found that LTM is associated with increased glial transcription and protein synthesis. Furthermore, we found that these increases are required for LTM formation. While increases in transcription and translation in neurons have been known to be important for LTM, this is the first indication that LTM also requires changes in glial transcription/translation. We are currently characterizing this requirement.
Thus far, we have identified a glial transcription factor, Repo, whose activity is required for LTM, and increases during LTM formation. In addition, we have identified dEAAT1, a factor regulated by Repo that is also required for LTM. dEAAT1 functions as a glutamate transporter, decreasing glutamate signaling at synapses, suggesting that decreased neuronal activity is required for consolidation of memory into LTM.
To determine how aging affects LTM, we are currently examining how aging affects training-dependent increases in Repo and dEAAT1. We have preminary data suggesting that old flies express less Repo and dEAAT1 compared to young flies. Furthermore, we have obtained supporting data suggesting that artificially increasing expression of Repo and dEAAT1 in old glial cells prevents age-related impairments in LTM.
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| 今後の研究の推進方策 |
We propose that age-related impairments in 1 hr memory and LTM are caused by impaired glial activity. Defects in 1 hr memory occur because old glial cells are unable to produce sufficient amounts of D-serine leading to decreased neuronal activity during memory acquisition. Defects in LTM are caused because old glial cells are unable to inhibit neuronal activity to memory consolidation, because they are unable to produce sufficient amounts of dEAAT1. We have three future goals. 1) We will demonstrate that training-dependent glial transcription is defective in old flies. 2) We will determine how neurons and glia communicate to regulate glial production of D-serine and dEAAT1 during memory formation. 3) We will determine the physiological consequences of decreased training-dependent glial expression in old flies.
1) We will measure training-dependent increases in transcription in young and old flies. We hypothesize that training-dependent increases in CREB-dependent neuronal transcription may not be affected by aging, while Repo-dependent increases in glial transcription will be reduced. 2) To identify neuronal/glial signaling processes, we will use both forward and reverse genetic screening strategies to identify neuronal and glial genes required for training-dependent increases in Repo activity. 3) To identify the physiological consequences of decreased Repo and dEAAT1 expression in old flies, we will determine the requirements of Repo and dEAAT1 in physiological processes such as sleep and neuronal viability during memory consolidation.
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| 次年度使用額が生じた理由 |
I intended to attend an international meeting at Cold Spring Harbor Laboratory, New York in the United States in September 2015, but could not attend for health reasons. The 300,513 yen remaining from my 2015 budget is because I missed this meeting.
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| 次年度使用額の使用計画 |
Since I was unable to attend the Cold Spring Harbor meeting last year, I will instead attend the Society for Neuroscience meeting at San Diego, USA in 2016. I will use my remaining budget from 2015 to attend this meeting.
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