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2016 年度 実施状況報告書

Thermodynamic basis of life: protein-carbohydrate interaction

研究課題

研究課題/領域番号 15K06962
研究機関九州大学

研究代表者

CAAVEIRO Jose  九州大学, 薬学研究院, 准教授 (00536732)

研究期間 (年度) 2015-04-01 – 2018-03-31
キーワードGlycan / Sugar / Antobody / Enzyme / Pore forming protein / X-ray crystallography / Thernodynamics / Kinetics
研究実績の概要

1. Research activities were advanced in different fronts with the goal of maximum productivity. Because I started a new laboratory at the University of Kyushu, in which I am the Principal Investigator, the research was concentrated in the publication of results. The experimentation had to to be slowed down momentarily and concentrated to essential aspects of the research. In particular, I have concentrated my efforts towards the determination of important crystal structures. In the fiscal year 2016/17 expenditures were reduced because of my transfer to Kyushu University. The money saved in 2016/17 will be used in fiscal year 17/18 to acquire essential scientific material to complete the project. I think this strategy is working really well, and has led to better than expected scientific output.
2. During the fiscal year 2016/17 I had the opportunity to present our research at various locations oversees. Importantly, the presentations were done by invitation. 3. I was also invited to Hokkaido University where I presented an important part of the results obtained in this project.
4. During the fiscal year 2016/17 the data of my research was published in various prestigious international journals, as detailed in the corresponding section.

現在までの達成度 (区分)
現在までの達成度 (区分)

1: 当初の計画以上に進展している

理由

Three invited talks (London by the Royal Society, and Chile), and seven papers is a extraordinary achievement for this project in my opinion.

今後の研究の推進方策

To obtain additional structural and thermodynamic data.
Publish 3-5 more papers, including one review.
Attend meetings in Japan and abroad.

次年度使用額が生じた理由

In 2016/17 I moved from The University of Tokyo to Kyushu University to start an independent research laboratory, in which I am the Principal Investigator. I have saved some money to be used on 2017/18 more efficiently in my new laboratory. This strategy has not slowed down the project in 2016/17.

次年度使用額の使用計画

The additional money will be used, entirely, for the purchase of goods and articles. This budget is essential for the completion of this project.

  • 研究成果

    (13件)

すべて 2017 2016 その他

すべて 雑誌論文 (7件) (うち国際共著 7件、 査読あり 7件、 オープンアクセス 3件、 謝辞記載あり 7件) 学会発表 (4件) (うち国際学会 1件、 招待講演 4件) 備考 (2件)

  • [雑誌論文] Disruption of cell adhesion by an antibody targeting the cell-adhesive intermediate (X-dimer) of human P-cadherin2017

    • 著者名/発表者名
      Kudo, S., Caaveiro, J.M.M., Nagatoishi, S., Miyafusa, T., Matsuura, T., Sudou, Y., and Tsumoto, K.
    • 雑誌名

      Sci. Rep.

      巻: 7 ページ: 39518

    • DOI

      10.1038/srep39518

    • 査読あり / オープンアクセス / 国際共著 / 謝辞記載あり
  • [雑誌論文] Peripheral membrane interactions boost the engagement by an anti HIV-1 broadly neutralizing antibody2017

    • 著者名/発表者名
      Rujas, E., Caaveiro, J.M.M., Insausti, S., Garcia-Porras, M., Tsumoto, K., and Nieva, J.L.
    • 雑誌名

      J. Biol. Chem.

      巻: 292 ページ: 5571-5583

    • DOI

      10.1074/jbc.M117.775429

    • 査読あり / 国際共著 / 謝辞記載あり
  • [雑誌論文] Functional contacts between MPER and the anti-HIV-1 broadly neutralizing antibody 4E10 extend into the core of the membrane2017

    • 著者名/発表者名
      Rujas, E., Insausti, S., Garcia-Porras, M., Sanchez-Eugenia, R., Tsumoto, K., Nieva, J.L., and Caaveiro, J.M.M.
    • 雑誌名

      J. Mol. Biol.

      巻: 429 ページ: 1213-1226

    • DOI

      10.1016/j.jmb.2017.03.008

    • 査読あり / 国際共著 / 謝辞記載あり
  • [雑誌論文] Identification of a membrane-bound prepore species clarifies the lytic mechanism of actinoporins2016

    • 著者名/発表者名
      Morante, K., Bellomio, A., Gil-Carton, D., Redondo-Morata, L., Sot, J., Scheuring, S., Valle, M., Gonzalez-Manas, J.M., Tsumoto, K., and Caaveiro, J.M.M.
    • 雑誌名

      J. Biol. Chem.

      巻: 291 ページ: 19210-19219

    • DOI

      10.1074/jbc.M116.734053

    • 査読あり / オープンアクセス / 国際共著 / 謝辞記載あり
  • [雑誌論文] Adhesive dimerization of human P-cadherin catalyzed by a chaperone-like mechanism2016

    • 著者名/発表者名
      Kudo, S., Caaveiro, J.M.M., and Tsumoto, K.
    • 雑誌名

      Structure

      巻: 24 ページ: 1523-1536

    • DOI

      10.1016/j.str.2016.07.002

    • 査読あり / 国際共著 / 謝辞記載あり
  • [雑誌論文] Differential effect of membrane composition on the pore forming ability of four different sea anemone actinoporins2016

    • 著者名/発表者名
      Garcia-Linares, S., Rivera-de-Torre, E., Morante, K., Tsumoto, K., Caaveiro, J.M.M., Gavilanes, J.G., Slotte, J.P., and Martinez-del-Pozo, A.
    • 雑誌名

      Biochemistry

      巻: 55 ページ: 6630-6641

    • DOI

      10.1021/acs.biochem.6b01007

    • 査読あり / 国際共著 / 謝辞記載あり
  • [雑誌論文] Structural basis for broad neutralization of HIV-1 through the molecular recognition of 10E8 helical epitope at the membrane interface2016

    • 著者名/発表者名
      Rujas, E., Caaveiro, J.M.M., Partida-Hanon, A., Gulzar, N., Morante, K., Apellaniz, B., Garcia-Porras, M., Bruix, M., Tsumoto, K., Scott, J.K., Jimenez, M.A., and Nieva, J.L.
    • 雑誌名

      Sci Rep.

      巻: 6 ページ: 38177

    • DOI

      10.1038/srep38177

    • 査読あり / オープンアクセス / 国際共著 / 謝辞記載あり
  • [学会発表] Thermodynamic properties of the transition state in biomolecular recognition phenomena2017

    • 著者名/発表者名
      Caaveiro, J.M.M.
    • 学会等名
      23rd Forum of Pharmaceutical Sciences, The Biophysical Society of Japan
    • 発表場所
      Sapporo, Japan
    • 年月日
      2017-03-16 – 2017-03-16
    • 招待講演
  • [学会発表] Biomolecular recognition: a structural and physicochemical perspective2016

    • 著者名/発表者名
      Caaveiro, J.M.M.
    • 学会等名
      Chile-Japan Academic Forum 2016 at Patagonia
    • 発表場所
      Puerto Natales, Chile
    • 年月日
      2016-11-07 – 2016-11-11
    • 招待講演
  • [学会発表] Thermodynamic tools in fragment-based drug discovery2016

    • 著者名/発表者名
      Caaveiro, J.M.M.
    • 学会等名
      2016 UT-PUCC Joint Workshop for Frontiers in Bioengineering and Biomedical Engineering
    • 発表場所
      Santiago, Chile
    • 年月日
      2016-11-04 – 2016-11-04
    • 招待講演
  • [学会発表] Pore formation assisted by lipids2016

    • 著者名/発表者名
      Caaveiro, J.M.M.
    • 学会等名
      Membrane pores: from structure and assembly, to medicine and technology, The Royal Society
    • 発表場所
      London, UK
    • 年月日
      2016-06-27 – 2016-06-27
    • 国際学会 / 招待講演
  • [備考] Issue cover and paper of the week in JBC

    • URL

      http://www.jbc.org/content/291/37.toc#PapersoftheWeek

  • [備考] Highligted by the Biophysical Society of Spain

    • URL

      http://biofisica.info/rujas-nieva-sci-rep-6-38177/

URL: 

公開日: 2018-01-16  

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