研究課題
1. Overall the project has surpassed the initial goals as I reason below, which leads me to affirm that this project has been a success. The scientific output until now comprises 15 papers and 11 presentations at international conferences and venues (invited, oral and posters), and it is very likely will increase as I publish more of the results obtained. This more than doubles the initial goal.2. The project was also extended to study the effect of glycan in additional models systems, like antibodies and human protein OMD in collaboration with Professor Kouhei Tsumoto from The University of Tokyo.3. The results show the importance of carbohydrates in a number of proteins, and dissect the energetic basis of their interaction. For example, we discovered that sugars can mimic lipids and recognize with low affinity a lipid-binding site. This could be an ancient mechanism of certain proteins to recognize a broad spectrum of cellular surfaces, since lipids and carbohydrates appear both on the external surface of cells.4. In antibodies, the glycan moiety attached to the Fc region has a very subtle but key importance for the recognition of the cellular receptor Fcgamma. Although the sugar does not contact the receptor it influences the binding of the antibody to it. How? The glycan regulates the flexibility of the antibody, with longer glycans reducing the entropy of the protein, leading to stronger affinity.
すべて 2018 2017
すべて 雑誌論文 (5件) (うち国際共著 5件、 査読あり 5件、 オープンアクセス 2件) 学会発表 (4件) (うち国際学会 1件、 招待講演 3件)
Sci. Rep.
巻: 8 ページ: 3955
10.1038/s41598-018-22199-8
J. Biochem.
巻: in press ページ: in press
10.1093/jb/mvy032
Commun. Biol.
巻: 1 ページ: 33
10.1038/s42003-018-0038-2
International Immunology
巻: 29 ページ: 311～317
10.1093/intimm/dxx038
Philos. Trans. R. Soc. Lond. B Biol. Sci.
巻: 372 ページ: 20160216
10.1098/rstb.2016.0216
