| 研究課題/領域番号 |
15K14608
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| 研究機関 | 東京工業大学 |
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研究代表者 |
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| 研究期間 (年度) |
2015-04-01 – 2018-03-31
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| キーワード | metalloenzyme / Electron microscopy / immunohistochemistry / bioelectrochemistry / Methane oxidation |
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| 研究実績の概要 |
Our collaborators have determined which multi-heme cytochrome proteins are expressed by ANME archaea. We have now designed antibodies against these same proteins, which should be finished within three months. After these are completed, we will apply them as initially described in the proposal.
In addition, we have summarized the current biochemical understanding of anaerobic methane oxidation in a review article which was published this year.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
3: やや遅れている
理由
Design of antibodies took longer than expected, but this has now been accomplished and they are ready to be made.
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| 今後の研究の推進方策 |
The plan remains the same as in the initial proposal. We are now making antibodies against ANME multiheme cytochromes and will apply them to cells to find out where they are located - on the inside, or outside of the cells.
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| 次年度使用額が生じた理由 |
Design of antibodies took longer than expected and they are ready to be made in FY2017.
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| 次年度使用額の使用計画 |
The remaining budget is planned to be used for the experiments in FY2017.
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