| 研究実績の概要 |
This study evaluated the alteration of cardiac beta-receptor signal using septic mouse model. We weighed the effects of dobutamine and milrinone as inotropes in BALB/c mice with cecal ligation and puncture (CLP)-induced sepsis. Surface expression levels of β1-adrenoceptors and alpha subunits of three main G protein families in myocardium were unaffected by CLP-induced sepsis. Plasma cyclic adenosine monophosphate (cAMP) levels were significantly elevated in both sham-operated and CLP mice in response to milrinone but only in sham-operated controls in response to dobutamine. Of phosphodiesterase (PDE) isoforms, PDE4D, but not PDE3A, both of which are responsible for cardiac cAMP hydrolysis, was significantly upregulated in CLP mouse myocardium.
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