| 研究実績の概要 |
To date this research has been going well, despite some initial setbacks that forced changes to the details of the experimental design. In examining patient samples we have looked at the two stromal markers that identify cancer associated fibroblasts (CD and alpha SMA) in a cohort of around 80 triple negative breast cancer patients, and are in the process of expanding this cohort by around an additional 50 patients. We then correlated the expression of these factors with the expression of androgen receptor and its associated enzymes to try and determine if stromal alterations govern changes in androgen signalling in this breast cancer subtype. In parallel we have been examining the interactions of AR and other nuclear receptors in the stromal as well as the epithelial compartments, as well as the presence of other indicators of altered cellular metabolism.
In parallel we have managed to characterize the cytokines secreted by two different patients stromal fibroblasts in response to two separate triple negative cell lines, to examine if the co-culture affects androgen metabolism and untangle underlying mechanisms.
On the strength of this data we are in the process of preparing the first manuscript to come from this work.
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