| 研究課題/領域番号 |
15K18558
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| 研究機関 | 筑波大学 |
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研究代表者 |
ブザス ディアナ・ミハエラ 筑波大学, 生命環境系, 准教授 (00616229)
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| 研究期間 (年度) |
2015-04-01 – 2018-03-31
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| キーワード | methylation |
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| 研究実績の概要 |
Computational analysis of DNA methylone in mutants of the FeS biogenesis pathway that localise to mitochondria revealed some interesting features about putative nuclear processes under control from mitochondria.
Mutants in new candidates from Fe-S biogenesis pathway that emerged from yeast studies are constructed to test their involvement in DNA demethyltion by DRE2.
Genome editing is being used to engineer DRE versions that may alter specifically part of the protein function.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
3: やや遅れている
理由
The transition to independent laboratory is a lot slower than expected. In addition, there are inherent technical issues associated with initial set up for experimental molecular biology.
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| 今後の研究の推進方策 |
Transgenics with modified, attenuated and inducible versions of the DRE2
protein in different cellular compartments will be created and profiled
for de-regulated gene sets, leading to elucidation of what biological
roles are connected to the nucleus-mithocondria communication pathway
disturbed in dre2-/-.
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| 次年度使用額が生じた理由 |
Fine adjustment was difficult to make
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| 次年度使用額の使用計画 |
Amount will contribute to research expenses in last year of the grant
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