| 研究実績の概要 |
In this study, we attempted to identify the woodwasp symbiont microbes from Hokkaido forest. We captured two wood wasp species, Sirex nitobei and Xiphydria cannelus. One bacteria and one fungus were isolated from a mygcangia of X. canelus, and were identified as Rahnella spp., and Daldinia decipiens species by 16s rRNA and ITS sequencing, respectively. Furthermore, we determined the hemicellulose-degrading activity in the supernatant when D. decipiens was cultured in the liquid medium containing polysaccharides such as cellulose, hemicellulose, wood biomass as sole carbon source, and glucose was used as a control culture. By utilizing proteomics, we were able to determined multiple glycoside hydrolase family enzymes, which are predicted to break down various polysaccharides present in woody biomass.
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