| 研究実績の概要 |
I have established the detection of gene expression of long non-coding RNA (lincRNA) H19 gene by real time RT-PCR and also established in situ hybridization method to check the type of cells in which these linc RNA were expressed using E7.5, E10.5, E13.5, E16.5 and E18.5 mouse placenta samples.
I presented about these findings in many conferences such as 22nd and 23rd meeting of Japan Placenta association conference (TO5-1), 120th Annual Meeting of The Japanese Association of Anatomists (P2-044), 121st 120th Annual Meeting of The Japanese Association of Anatomists (3OpmF-2) and 68th Annual Congress of the Japan Society of Obstetrics and Gynecology (P2-35-3).
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
After established real-time RT-PCR and in situ hybridization methods, I am now analyzing new possible candidate of placenta-specific lincRNAs.
Then, I will continue the functional analysis of lncRNA by placenta-specific gene manipulation of mouse using in vitro fertilization.
I presented my new findings in many conferences.
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| 今後の研究の推進方策 |
I will continue functional analysis of placenta-specific lncRNA that varies in miscarriage placenta, using RNAi by in vitro fertilization of the mouse.
1)Placenta-specific lncRNA that varies in miscarriage placenta: I will continue to extract total RNA from miscarriage placenta and normal pregnant women. To identify miscarriage-associated lncRNA, I will perform the same method used in mice. I can estimate the reduced or increased gene expression level and setting up gene expression control network of lncRNAs involved in miscarriage using IPA software (Ingenuity, Inc. Installed in our department).
2) Functional analysis of lncRNA using human placental trophoblast-derived cell lines, BeWo cells: A major function of lncRNAs is to control gene degradation and promotion and inhibition of gene expression in human placental trophoblast-derived cell lines, BeWo cells. Therefore, knocked down the lncRNA that varies in miscarriage by RNAi in BeWo cells, and then analyzed changes in the gene expression profile, cell growth, and apoptosis.
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