| 研究課題/領域番号 |
15K20949
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| 研究機関 | 東京大学 |
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研究代表者 |
頼 貞儀 東京大学, 医科学研究所, 特任研究員 (30739925)
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| 研究期間 (年度) |
2015-04-01 – 2017-03-31
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| キーワード | Autoinflammation / CGD |
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| 研究実績の概要 |
Chronic granulomatous disease (CGD) is basically a phagocyte disorder, but is also characterized by autoinflammation in gastrointestinal tract and it remains to be the problem. We hypothesize that innate lymphoid cells (ILCs) may play roles on CGD colitis. To verify this, first, the hematopoietic stem and progenitor cells (HSPCs), the origin of ILCs, was examined in a X-CGD murine model. Results showed HSPCs in CGD mice comparable to control, suggesting that progenitor cell differentiation remained unaffected. Second, the induced pluripotent stem (iPS) cells derived from a CGD patient were established. The results obtained from mouse model will be translated to human CGD-iPS cells, it is of important since we are aiming at establishment of treatment for CGD colitis via regulation of ILCs in patients.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
3: やや遅れている
理由
The research was slightly delayed for the reason that production of X-CGD mice did not works so well due to the aging of the carrier female. To deal with this, the mating strategy was switched to producing the new generation carrier females by using male X-CGD mice and wild-type female mice.
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| 今後の研究の推進方策 |
The research will be carried as previously planed once the new generation X-CGD mice become available. Even through the progress in FY2015 was slightly delayed due to the lack of X-CGD mice, the production of human CGD-iPS cells which was planed to be established in FY2016 was done in advance. Therefore, during the waiting period of X-CGD mice production, the downstream experiments using CGD-iPS cells will also proceed simultaneously.
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| 次年度使用額が生じた理由 |
The reason for incurring FY2015 research funding to the next year is that the research progress was slightly delayed due to the production of X-CGD mice did not work so well. Therefore, only a part of the funding in FY2015 was used.
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| 次年度使用額の使用計画 |
The incurred amount of funding will be used to purchase the experimental animals and materials as initially planned.
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