| 研究課題/領域番号 |
16F16386
|
|---|
| 研究機関 | 国立研究開発法人理化学研究所 |
|---|
研究代表者 |
McHugh Thomas 国立研究開発法人理化学研究所, 脳科学総合研究センター, チームリーダー (50553731)
|
|---|
| 研究分担者 |
CHEN SHUO 国立研究開発法人理化学研究所, 脳科学総合研究センター, 外国人特別研究員
|
|---|
| 研究期間 (年度) |
2016-10-07 – 2018-03-31
|
| キーワード | hypothalamus / hippocampus / DG / spatial working memory |
|---|
| 研究実績の概要 |
Optogenetic stimulation of SuM terminals in the DG does not impair social behavior, however we have found that inhibition of these projections leads to profound deficits in spatial working memory, while stimulation of the terminals impaired context habituation. In vivo recordings of hippocampal physiology during T-maze behavior combined with optogenetics suggests that silencing of this input impairs trajectory-specific firing rate modulation of CA1 pyramidal cells in a manner that correlates with an increase in error trials. These data establish the SuM inputs as a novel modulator of hippocampal spatial memory and we are currently conducting analyses to link the behavioral and single unit phenotypes to alterations in hippocampal oscillations and dynamic information routing in the circuit.
|
| 現在までの達成度 (区分) |
現在までの達成度 (区分)
1: 当初の計画以上に進展している
理由
The project has developed several interesting lines of inquiry. The main focus on the role of the SuM/Dg circuit in spatial working memory has lead to clear behavioral and physiological phenotypes. In related work we have begun to examine the SuM projections to the medial septum and have striking and differential data showing that optogenetic stimulation of these fibers induces high speed movement and triggers a strong increase in the theta oscillation. these unexpected data will lead to a parallel series of experiments.
|
| 今後の研究の推進方策 |
We are currently focused on adding more In vivo recordings of hippocampal physiology during manipulation of the SuM fibres in both the DG and medial septum. The effort this fiscal year will be to complete data collection and begin detailed analyses.
|
