| 研究課題/領域番号 |
16F16397
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| 研究機関 | 島根大学 |
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研究代表者 |
浅尾 俊樹 島根大学, 生物資源科学部, 教授 (30252901)
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| 研究分担者 |
ASADUZZAMAN MD. 島根大学, 生物資源科学部, 外国人特別研究員
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| 研究期間 (年度) |
2016-11-07 – 2019-03-31
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| キーワード | Strawberry / lettuce / micropropagation / bioassay / oxidative damage / antioxidant responses |
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| 研究実績の概要 |
In the first part of the fellowship, oxidative damage and antioxidant responses in strawberry due to benzoic acid application was scheduled. Achievements so far includes-
Preparation of plant materials and bioassay with strawberry plantlets:Apex culture: In order to produce pathogen free, similar growth rates and vigor strawberry plantlets, apex culture of strawberry runner were done. Micropropagation: In vitro strawberry plantlets were prepared and currently at multiplication stage.
Bioassay of exogenous benzoic acid application with lettuce seedlings:Lettuce is a sensitive plant species and we used it in our pre-experiment plant material to study the root oxidative damage and antioxidant responses due to allelochemical (Benzoic acid) application.Lettuce were sown in urethane foam directly and placed in the growth chapter at 20/20 °C (day/night), 12 hrs day length. In order to encourage root formation, lettuce seedlings were transferred to nursery condition with 50% Enshi-shoho nutrient solution and aeration. Plastic containers with 3 L nutrient solution were used for this bioassay. Ten seedlings were planted in each container.
Preparation of bioassay and antioxidant reactions analysis using strawberry plantlets:Plant materials (strawberry plantlets) and experimental materials were prepared. Bioassay and physical damage of roots, physiological and biochemical reactions (antioxidant enzymatic reactions) due to benzoic acid application in culture solution is under investigation.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
3: やや遅れている
理由
In this research period, the fellow took temporary leave of absence during 26/12/2016 to 28/2/2017. He returned to Bangladesh in order to complete his on-going research programs in Bangladesh Agricultural Research Institute. Therefore, the research schedules under this program slightly modified and changed.
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| 今後の研究の推進方策 |
Several experiments will be conducted to study the physiological responses and biochemical activities and expression of antioxidant enzymes in strawberry root under non-renewed culture.
In the experiment bioassay will be carried out using strawberry plantlets. Strawberry plantlets will be grown in 25% Enshi nutrient solution at 0, 1, 5, 10, 25, 50, 100, 200 and 400 micro M/L benzoic acid. Plantlets will be grown for two weeks and root samples will be collected for the following observation and antioxidative analysis. i. Observation of lettuce cell integrity under Scanning Electron Microscope: ii. Determination of NADPH oxidase activity of plasma membranes. iii. Antioxidant enzyme activity and reactive oxygen species (ROS) determination. iv. Total superoxide dismutase activity. v. The activity of guaiacol peroxidase and Catalase. vi. Ascorbate peroxidase activity. vii. O2- activity. viii. Determination of lipid peroxidate and plasma membrane H+-ATPase activity measurement. ix. The plasma membrane- enriched and tonoplast - enriched vesicles. x. The activity of H+-ATPase. xi. In vivo determination of ROS and root viability.
In further experiment, strawberry plant will be cultured in nutrient solution either renewed or non-renewed. The concentrations of the main nutrients in the culture solution will be adjusted every three weeks. Strawberry plants will be cultured until root become brown to black in order to evidence the allelochemical stress in non-renewed culture solution. The root samples will be collected for further analysis as described above in the bioassay.
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