寿命と発生を制御するシグナル伝達ネットワーク

2008 年度 研究成果報告書

• 研究課題/領域番号: 16GS0310
• 研究種目: 学術創成研究費
• 配分区分: 補助金
• 研究機関: 京都大学
• 研究代表者: 西田 栄介 京都大学, 大学院・生命科学研究科, 教授 (60143369)
• 研究期間 (年度): 2004 – 2008
• キーワード: シグナル伝達 / 寿命 / 発生

研究概要

線虫において断続的飢餓が著しく寿命を促進し、Rheb 経路がその寿命促進に必要であることを発見した。脊椎動物初期胚発生過程の背腹軸決定において、フィードバックインヒビターSprouty によるERK 経路の活性化時間の制御が必須であることを発見した。ERK の核内での作用機構について解析し、ERK 依存的な遺伝子の転写促進が起こる場合に、その遺伝子の近傍領域の転写も活性化する「転写の波及効果」を発見した。

研究成果

• [雑誌論文] Signalling through Rheb mediates intermittent fasting-induced longevityin C. (2009)
  • 著者名/発表者名: Honjoh, S., Yamamoto, T., Uno, M. and Nishida, E.
  • 雑誌名: elegans. Nature 457 ページ: 726-730
• [雑誌論文] Regulation of the ERK activity duration by Sprouty contributes to dorsoventral patterning (2009)
  • 著者名/発表者名: Hanafusa, H., Matsumoto, K. and Nishida, E.
  • 雑誌名: Nature Cell Biol 11 ページ: 106-109
• [雑誌論文] Ripples from neighbouring transcription (2008)
  • 著者名/発表者名: Ebisuya, M., Yamamoto, T., Nakajima, M. and Nishida, E.
  • 雑誌名: Nature Cell Biol 10 ページ: 1106-1113
• [雑誌論文] FGF induces oscillations of Hes1 expression and Ras/ERK activation (2008)
  • 著者名/発表者名: Nakayama, K., Satoh, T., Igari, A., Kageyama, R. and Nishida, E.
  • 雑誌名: Curr. Biol 18 ページ: R332-R334
• [雑誌論文] Myt1 protein kinase is essential for Golgi and ER assenmbly during mitotic exit (2008)
  • 著者名/発表者名: Nakajima, H., Yonemura, S., Murata, M., Nakamura, N., Piwnica-Worms, H. and Nishida, E.
  • 雑誌名: J. Cell Biol 181 ページ: 89-103
• [雑誌論文] PtdIns(3, 4, 5)P3 regulates spindle orientation in adherent cells (2007)
  • 著者名/発表者名: Toyoshima, F., Matsumura, S., Morimoto, H., Mitsushima, M. and Nishida, E.
  • 雑誌名: Dev. Cell 13 ページ: 796-811
• [雑誌論文] Integrin-mediated adhesion orients the spindle parallel to the substratum in an EB1-and myosin X-dependent manner (2007)
  • 著者名/発表者名: Toyoshima, F., and Nishida, E.
  • 雑誌名: EMBO J 26 ページ: 1487-1498
• [雑誌論文] Continuous ERK activation downregulates antiproliferative genes throughout G1 phase to allow cell-cycle progression (2006)
  • 著者名/発表者名: Yamamoto, T., Ebisuya, M., Ashida, F., Okamoto, K., Yonehara, S. and Nishida, E.
  • 雑誌名: Curr. Biol 16 ページ: 1171-1182
• [雑誌論文] MAPK signalling : ERK5 versus ERK1/2 (2006)
  • 著者名/発表者名: Nishimoto, S. and Nishida, E.
  • 雑誌名: EMBO Rep 7 ページ: 782-786
• [雑誌論文] Requirement of the MAP kinase signaling pathways for mouse preimplantation development (2005)
  • 著者名/発表者名: Maekawa, M., Yamamoto, T., Tanoue, T., Yuasa, Y., Chisaka, O. and Nishida, E.
  • 雑誌名: Development 132 ページ: 1173-1783
• [雑誌論文] Requirement of the MEK5-ERK5 pathway for neural differentiation in Xenopus embryonic development (2005)
  • 著者名/発表者名: Nishimoto, S., Kusakabe, M. and Nishida, E.
  • 雑誌名: EMBO Rep 6 ページ: 1064-1069
• [雑誌論文] The polarity-inducing kinase Par-1 controls Xenopus gastrulation in cooperation with 14-3-3 and aPKC (2004)
  • 著者名/発表者名: Kusakabe, M. and Nishida, E.
  • 雑誌名: EMBO J 23 ページ: 4190-4201
• [雑誌論文] Sef is a spatial regulator for Ras/MAP kinase signaling (2004)
  • 著者名/発表者名: Torii, S., Kusakabe, M., Yamamoto, T., Maekawa, M. and Nishida, E.
  • 雑誌名: Dev. Cell 7 ページ: 33-44
• [雑誌論文] ERK activation propagates in epithelial cell sheets and regulates their migration during wound healing (2004)
  • 著者名/発表者名: Matsubayashi, Y., Ebisuya, M., Honjoh, S. and Nishida, E.
  • 雑誌名: Curr. Biol 14 ページ: 731-735
• [学会発表] Serum- and glucocorticoid-inducible kinase 1 (SGK1) promotes cell survival by suppressing the expression of death-inducing signaling complex (DISC) components in early Xenopus development (2008)
  • 著者名/発表者名: Endo, T., Kusakabe, M., Sunadome, K., Yamamoto, T. and Nishida, E.
  • 学会等名: The 12th International Xenopus conference
  • 発表場所: Eurostrand Hotel, Leiwen, Germany
  • 年月日: 20080908-12
• [学会発表] Functional analysis of EIG121L (estrogen induced gene 121-like) in early Xenopus development (2008)
  • 著者名/発表者名: Araki, T., Kusakabe, M. and Nishida, E.
  • 学会等名: The 12th International Xenopus Conference
  • 発表場所: Eurostrand Hotel, Leiwen, Germany
  • 年月日: 20080908-12
• [学会発表] Antagonistic Interactions between the Retinoic Acid Receptor and Ras/ERK Signaling Pathways in Colorectal Cancer Cells (2007)
  • 著者名/発表者名: Imajo, M., Kondoh, K., Yamamoto, T., Nakayama, K. and Nishida, E.
  • 学会等名: The 47th Annual Meeting of the American Society for Cell Biology
  • 発表場所: Washington Convention Center, Washington DC, USA
  • 年月日: 20071201-05
• [学会発表] Rheb is required for intermittent fasting-induced life span extension (2007)
  • 著者名/発表者名: Honjoh, S. and Nishida, E.
  • 学会等名: 16th International C
  • 発表場所: elegans meeting University of California
  • 年月日: 20070626-0701
• [学会発表] A screen for genes comprising C. elegans MAPK cascades that affect the lifespan (2006)
  • 著者名/発表者名: Okuyama, T., Kano, K., Ookuma, S., Inoue, H., Hisamoto, N., Matsumoto, K. and Nishida, E.
  • 学会等名: The 2nd East Asia C. elegans Meeting
  • 発表場所: The Hoam Convention Center, Seoul National University, Seoul, Korea
  • 年月日: 20061115-18
• [学会発表] Signal transduction by the ERK family of MAP kinases (2006)
  • 著者名/発表者名: Nishida, E
  • 学会等名: International Symposium of Kobe University 21st century COE program on Signal transduction In Memory of Prof. Yasutomi Nishizuka
  • 発表場所: International Conference Center Kobe, Japan
  • 年月日: 20060209-11
• [学会発表] The MEK5-ERK5 pathway is essential for Xenopus neural differentiation (2005)
  • 著者名/発表者名: Nishimoto, S., Kusakabe, M. and Nishida, E.
  • 学会等名: 15th International Society of Developmental Biologists Congress 2005
  • 発表場所: Sydney, Australia
  • 年月日: 20050903-07
• [学会発表] Extension of the lifespan of Caenorhabditis elegans by beta-the lifespan of Caenorhabditis elegans by betacarotene (2005)
  • 著者名/発表者名: Ookuma, S., Taniguchi, E., Bamba, C., Kanoh, K., Hashimoto, Y. and Nishida, E
  • 学会等名: 15th International C. elegans Conference
  • 発表場所: University of California, Los Angeles, California, USA
  • 年月日: 20050625-29
• [学会発表] Signal transduction by the ERK family of MAP kinases (2004)
  • 著者名/発表者名: Nishida, E
  • 学会等名: The First Shanghai Symposium On Signal Transduction And Cancer
  • 発表場所: Shanghai, China
  • 年月日: 20040829-0901