| 研究実績の概要 |
During FY[2018], the platform was further improved:
1- Devices with an improved design were used; 2- A small warm chamber was designed to keep the neurons on the paltform at 37C during experiments; 3- As neurons activity is weak, improvement of the signal to noise ratio with new types of connections and set-up was performed; 4- Various improvement of the neurons culture was performed to avoid dying or inactivation of the cells.
Countrary to what was announced, muscle cells were not available in our partner laboratory. However, cortical neurons were cultured and grown as organoids (like micro-brain), and activity could be measured successfully in 2D. A new research project to study organoids communication will be proposed. In parallel, cardiomyocyte cells were cultured on the device and their activity in 2D was successfully measured. Here too, a new project is in elaboration, in which the motor neuron / cardiomyocyte system will be studied. Finally, integration of the platform with a biomimetic stimulation system was performed, and will be presented in ISMM'2019.
As a conclusion, we can say that thanks to that Kakenhi Kiban B grant, a platform for electrogenic cells activity 2D measurement and study, combined to a SNN system for biomimetic stimulation, has been developed, and will be used for new projects in the area of biomedical applications.
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