研究課題/領域番号 |
16H06197
|
研究機関 | 国立研究開発法人森林研究・整備機構 |
研究代表者 |
Worth James 国立研究開発法人森林研究・整備機構, 森林総合研究所, 主任研究員 等 (30770771)
|
研究期間 (年度) |
2016-04-01 – 2019-03-31
|
キーワード | Tsuga diversifolia / Tsuga sieboldii / Fagus crenata / NGS / SSR markers / population genetics |
研究実績の概要 |
I designed nuclear and chloroplast SSR markers for Japanese Tsuga species. Using these I have genotyped 35 populations for each of Tsuga sieboldii and T. diversifolia from across their ranges. No evidence of hybridisation was observed between the species. There was significant genetic structuring of genetic diversity in both species. For Fagus crenata I have assembled the whole chloroplast genome. A phylogenetic tree based on whole chloroplast genomes of the major haplotypes in Fagus crenata showed two diverged clades in western and eastern Japan. Unexpectedly the Chinese F. engeleriana found to be nested within the western F. crenata clade. I have also obtained RAD-seq data from 354 samples of Fagus crenata sampled from 4 altitudinal clines.
|
現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
I have been able to achieve my goals for the 2nd year of this project.
|
今後の研究の推進方策 |
I will publish a paper on the genetic diversity and structure of Japanese Tsuga species based on nuclear and chloroplast SSR markers. I also plan to complete the analysis of RAD-seq data to identify candidate SNPs under selection for temperature across 4 independent altitudinal clines and publish a paper. This will be aided by the soon to released whole genome sequence of F. crenata. Lastly, I plan to complete sampling of Thuja sieboldii and develop nuclear SSR markers to discover how long small isolated populations in western Japan at the warm edge of the species range have persisted.
|