| 研究実績の概要 |
Physical exercise enhances fitness and overall health and wellness. Bouts of aerobic and anaerobic exercise induce an acute state of oxidative stress caused by an increase in reactive oxygen species (ROS), but there is little agreement which processes generate ROS and where, if they are important and how they are controlled. We produced transgenic mice expressing redox-sensitive GFP in skeletal muscle, and set up imaging systems to record muscle redox state in live anesthetized animals. Determining the conditions necessary for induction of oxidative stress during skeletal muscle activation in mice in vivo, preliminary experiments indicated that some amount of muscle fiber injury is necessary to induce oxidative stress during skeletal muscle activation. Simple electrical stimulation of muscle did not induce oxidation of the skeletal muscle glutathione pool. Injured muscle fibers became oxidized, but this oxidation did not spread to adjacent fibers in the absence of muscle activation.
Experiments to publish our finding that muscle activation without injury does not result in oxidative stress were finished, but experiments using controlled injuries did not yield satisfactory results, and alternative strategies are under consideration.
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