2019 年度実績報告書

Studies on influenza A virus M2-host interactome and its role in virus replication

研究課題

研究課題/領域番号	16K08014
研究機関	北海道大学
研究代表者	マンズールラシッド北海道大学, 人獣共通感染症リサーチセンター, 特任助教 (90566150)
研究期間 (年度)	2016-04-01 – 2020-03-31
キーワード	Influenza A virus / M2 / Host interaction
研究実績の概要	The M2 protein is involved in various steps of viral replication and also affects the host cellular functions in ion channel activity-dependent and -independent manners. While en route to the cell membrane, the M2-ectodomain and cytoplasmic tail are exposed to vesicular/organelle and cytoplasmic environments, where they may interact with various cellular proteins.During this study we identified host-proteins which interact with IAV M2 protein and affect virus replication. We found two amino acid residues (position 54 and 57) uniquely different between two IAV subtypes governed the interaction of M2 with plasma membrane thereby playing role in virus budding/release. The M2 protein, besides increasing the transmembrane domain (TMD) length and having two inherent lipid raft targeting features, did not associate with lipid rafts suggesting that the M2-TMD length is not the only non-lipid microdomain localization determinant. We also found that virus release/budding required optimum length of M2-TMD.

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