| 研究実績の概要 |
The aim of the present study was to determine the amplification status of 26 genes, which are frequently amplified in solid cancers, in advanced gastric cancers. A total of 93 formalin-fixed and paraffin-embedded advanced gastric cancer tissues were examined by multiple ligation-dependent probe amplification (MLPA), and 32 cases with ‘gain’ or ‘amplified’ status of 16 genes were further examined for the respective gene amplification by fluorescence in situ hybridization (FISH). The frequencies of gene amplifications in these gastric cancers as assessed by these combined methods were as follows: ERBB2 (14%), FGFR2 (7.5%), MYC (7.5%), TOP2A (7.5%), MET (4.3%), MDM2 (4.3%), CCND1 (3.2%), FGF10 (3.2%), and EGFR (1.1%). Attempts at correlating the FISH status with the immunohistochemical staining pattern showed variable results. In conclusion, combination of MLPA and FISH analysis is a feasible approach for obtaining the semi-comprehensive genetic information that is necessary for personalized molecular targeted therapy.
以上の結果はHuman Pathology (2017) 61: 58-67 に” Gene amplification of CCNE!, CCND1, and CDK6 in gastric cancers detected by multiplex ligation-dependent probe amplification and fluorescence in situ hybridization” に誌上発表した。
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