| 研究実績の概要 |
An archive of 322 formalin-fixed and paraffin-embedded invasive ductal cancer tissues were screened by multiple ligation-dependent probe amplification and, a total 885 gene loci judged as ‘gain’ or ‘amplified’ concerning the panel of the 22 genes was further examined for the respective gene amplification by fluorescence in situ hybridization. The results showed 111 of 322 tumors (35%) displayed gene amplification of at least one of the 22 genes. The frequencies of the amplification of four regions with known driver oncogenes were as follows: 8p11 (ZNF703, FGFR1, ADAM9 and IKBKB), 8q24 (MTDH, MYC), 11q13 (CCND1, C11ORF30), and 17q11-21 (CPD, MED1, ERBB2, CDC6, TOP2A, MAPT) were 10%, 12.7%, 12.4%, and 12.7%, respectively. In addition to homogeneously staining region-type or double minute chromosome-type amplifications, the centromere-associated type amplification in nine tumors. Co-localization of the amplicon on 8p11 and the amplicon on 11q13 in single cells was found in 10 tumors, and in the six of them the two amplicons constituted single amplification units. Similarly, amplicon consisting ERBB2 and its franking genes on 7q12-21 co-localized with the amplicon on 8p11 in 10 tumors and with the amplicon on 11q13 in five tumors.
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