| 研究実績の概要 |
This study aims to identify host responses in whole blood of children infected with respiratory syncytial virus (RSV) using omics approach. Identified host factors will be useful as biomarkers in distinguishing mild vs. severe pneumonia.Approval from ethics committee were obtained at Tohoku University Graduate School of Medicine, Sendai Medical Center, and Miyagi Children’s Hospital. Pilot experiments were successfully performed including collection of peripheral blood samples, isolation and purification of total RNA and proteins.
We enrolled a total of 39 children at Sendai Medical Center and Miyagi Children’s Hospital. Whole blood samples and nasopharyngeal swabs were collected from patients followed by total RNA isolation and purification. PCR was performed on nasopharyngeal swabs and identified 19 RSV, 7 rhinovirus, 2 human metapneumovirus, 1 influenza B virus, 1 adenovirus, and 9 negative. Clinical data were obtained and patients were grouped into mild and severe groups according to 5 parameters such as respiratory rate, wheezing, cyanosis, accessory respiratory muscle utilization, and oxygen saturation.
Whole blood transcription profiling by next-generation sequencing is currently ongoing. Proteome profiling of patients by liquid chromatography-mass spectrometry is planned in the future.
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