• 研究課題をさがす
  • 研究者をさがす
  • KAKENの使い方
  1. 課題ページに戻る

2016 年度 実施状況報告書

Compression stress induced deformation of microtubule on an elastic medium

研究課題

研究課題/領域番号 16K16383
研究機関北海道大学

研究代表者

コビル アリフ  北海道大学, 理学研究院, 特任助教 (10724867)

研究期間 (年度) 2016-04-01 – 2018-03-31
キーワードMicrotubule / Mechanical deformation / Buckling / Compression stress / Elastic medium
研究実績の概要

In the last year, in order to demonstrate compression stress induced mechanical deformation of microtubules in vitro, we have developed a computer controlled mechanical chamber system.The main component of the chamber system is a mechanical chamber equipped with DC motors, controller, and other electronic accessories. Using the mechanical chamber system, we have performed preliminary investigations on the response of microtubules to compression stress. We demonstrated compression test of microtubules in vitro by placing the microtubules on a soft elastomeric material PDMS. Kinesin was used an an anchor to fix the microtubules to the PDMS. The PDMS served here as an elastic medium similar to the environment of microtubules in cell, where cytoplasm serves as an elastic medium.
Upon application of compression stress to the microtubules using the mechanical chamber buckling of microtubules, akin to that observed in cell has been reproduced in laboratory. Role of interaction of microtubules with surrounding elastic medium has also been investigated with the help of computer simulation, and compared with the experimental results. From our investigations, it has been revealed that extent of interaction of microtubules with the surrounding elastic medium strongly influences the buckling mode and extent of buckling of microtubules. In addition, strain has a significant role, but strain rate has no influence on the buckling of microtubules.

現在までの達成度 (区分)
現在までの達成度 (区分)

2: おおむね順調に進展している

理由

So far progress of the project has been satisfactory in the last year. According to our proposed plan, we have developed a computer controlled mechanical chamber system. Performance of the chamber system has been authenticated by demonstrating compression stress induced mechanical deformation of microtubules in vitro. The deformation closely mimicked the deformation mode of microtubules in cell. Therefore, the challenges for the first year were successfully overcome and the targets have been mostly achieved.

今後の研究の推進方策

In the next year, detail investigation on the compression stress induced mechanical deformation of microtubules will be carried out. Effect of an elastic medium around microtubules and interaction mode of microtubules with the medium on the deformation of microtubules will be vividly investigated. In addition, role of microtubule associated protein kinesin, strain, etc. in the deformation of microtubules will be thoroughly investigated and compared with the deformation of microtubules in cells.

次年度使用額が生じた理由

In the last year the project has been partially implemented. In the current year the aim is to complete the project as was proposed. So a considerable amount of work has to be done in this year, for which it would be required to purchase many materials.

次年度使用額の使用計画

The mechanical chamber system has already been developed in the last year. So in the current year mainly it might be required to purchase various chemicals and reagents for performing experiments. So the plan is to consume the budget for mainly purchase of the required chemicals and reagent materials.

  • 研究成果

    (21件)

すべて 2017 2016

すべて 雑誌論文 (2件) (うち国際共著 2件、 査読あり 2件、 謝辞記載あり 1件、 オープンアクセス 1件) 学会発表 (19件) (うち国際学会 5件、 招待講演 2件)

  • [雑誌論文] Buckling of microtubules on elastic media via breakable bonds2016

    • 著者名/発表者名
      Tanjina Afrin, Arif Md. Rashedul Kabir, Kazuki Sada, Akira Kakugo, Takahiro Nitta
    • 雑誌名

      Biochemical and Biophysical Research Communications (BBRC)

      巻: 480 ページ: 132-138

    • DOI

      10.1016/j.bbrc.2016.09.133

    • 査読あり / 国際共著 / 謝辞記載あり
  • [雑誌論文] Sensing surface mechanical deformation using active probes driven by motor proteins2016

    • 著者名/発表者名
      Daisuke Inoue, Takahiro Nitta, Arif Md. Rashedul Kabir, Kazuki Sada, Jian Ping Gong, Akihiko Konagaya, Akira Kakugo
    • 雑誌名

      Nature Communications

      巻: 7 (12557) ページ: 1-10

    • DOI

      10.1038/ncomms12557

    • 査読あり / オープンアクセス / 国際共著
  • [学会発表] Biomolecular motor powered molecular robots2017

    • 著者名/発表者名
      Arif Md. Rashedul Kabir, Akira Kakugo
    • 学会等名
      Workshop on molecular robotics
    • 発表場所
      Koshiba Hall, Hongo Campus, University of Tokyo, Bunkyo-ku, Tokyo
    • 年月日
      2017-03-11
  • [学会発表] Behavior of microtubule collective motion induced by depletion force2017

    • 著者名/発表者名
      Ai Saito, Ryuhei Suzuki, Tamanna Ishrat Farhana, Arif Md. Rashedul Kabir, Kazuki Sada, Akira Kakugo
    • 学会等名
      Winter meeting, Hokkaido Branch, SPSJ
    • 発表場所
      Hokkaido University, Sapporo, Hokkaido
    • 年月日
      2017-01-19
  • [学会発表] Buckling of Microtubules on an Elastic Medium2016

    • 著者名/発表者名
      Arif Md. Rashedul Kabir, Tanjina Afrin, Kazuki Sada, Akira Kakugo
    • 学会等名
      The 17th RIES-HOKUDAI INTERNATIONAL SYMPOSIUM
    • 発表場所
      Chateraise Gateaux Kingdom, Sapporo, Hokkaido
    • 年月日
      2016-12-13 – 2016-12-14
    • 国際学会
  • [学会発表] Dynamic Behavior of Swarm Microtubules Driven by Kinesins2016

    • 著者名/発表者名
      Arif Md. Rashedul Kabir
    • 学会等名
      The 6th Soft Matter Symposium
    • 発表場所
      Hokkaido University, Sapporo, Hokkaido
    • 年月日
      2016-10-24 – 2016-10-26
    • 招待講演
  • [学会発表] Mechanical Deformation Induced Modulation of Biochemical Functions of Microtubules2016

    • 著者名/発表者名
      Tanjina Afrin, Arif Md. Rashedul Kabir, Kazuki Sada, Akira Kakugo
    • 学会等名
      The 6th Soft Matter Symposium
    • 発表場所
      Hokkaido University, Sapporo, Hokkaido
    • 年月日
      2016-10-24 – 2016-10-26
  • [学会発表] Collective Motion of Microtubules with Different Lengths and Rigidity2016

    • 著者名/発表者名
      Tamanna Ishrat Farhana, Arif Md. Rashedul Kabir, Daisuke Inoue, Kazuki Sada, Akira Kakugo
    • 学会等名
      The 6th Soft Matter Symposium
    • 発表場所
      Hokkaido University, Sapporo, Hokkaido
    • 年月日
      2016-10-24 – 2016-10-26
  • [学会発表] Response behavior of microtubules to external mechanical stimuli2016

    • 著者名/発表者名
      Seiji Nishikawa, Tanjina Afrin, Arif Md. Rashedul Kabir, Kazuki Sada, Akira Kakugo
    • 学会等名
      The 6th Soft Matter Symposium
    • 発表場所
      Hokkaido University, Sapporo, Hokkaido
    • 年月日
      2016-10-24 – 2016-10-26
  • [学会発表] Controlling the direction of microtubule assembly using photoresponsive DNA2016

    • 著者名/発表者名
      Ryuhei Suzuki, Daisuke Inoue, Arif Md. Rashedul Kabir, Kazuki Sada, Akinori Kuzuya, Hiroyuki Asanuma, Akira Kakugo
    • 学会等名
      The 6th Soft Matter Symposium
    • 発表場所
      Hokkaido University, Sapporo, Hokkaido
    • 年月日
      2016-10-24 – 2016-10-26
  • [学会発表] DNA assisted control of swarming of a biomolecular motor system2016

    • 著者名/発表者名
      Jakia Jannat Keya, Arif Md. Rashedul Kabir, Daisuke Inoue, Kazuki Sada, Akinori Kuzuya, Akira Kakugo
    • 学会等名
      The 6th Soft Matter Symposium
    • 発表場所
      Hokkaido University, Sapporo, Hokkaido
    • 年月日
      2016-10-24 – 2016-10-26
  • [学会発表] Reproduction and motility control of cilia structure in vitro2016

    • 著者名/発表者名
      Ren Sasaki, Shoki Wada, Daisuke Inoue, Arif Md. Rashedul Kabir, Kazuki Sada, Akira Kakugo
    • 学会等名
      The 6th Soft Matter Symposium
    • 発表場所
      Hokkaido University, Sapporo, Hokkaido
    • 年月日
      2016-10-24 – 2016-10-26
  • [学会発表] Mechanical Oscillation of Dynamic Microtubule Rings2016

    • 著者名/発表者名
      Masaki Ito, Arif Md. Rashedul Kabir, Md. Sirajul Islam, Daisuke Inoue, Shoki Wada, Kazuki Sada, Akihiko Konagaya, Akira Kakugo
    • 学会等名
      The 6th Soft Matter Symposium
    • 発表場所
      Hokkaido University, Sapporo, Hokkaido
    • 年月日
      2016-10-24 – 2016-10-26
  • [学会発表] Regulatory Effects of ROS on the Dynamic Instability of Microtubules2016

    • 著者名/発表者名
      Md. Sirajul Islam, Arif Md. Rashedul Kabir, Daisuke Inoue, Kazuki Sada, Akira Kakugo
    • 学会等名
      The 6th Soft Matter Symposium
    • 発表場所
      Hokkaido University, Sapporo, Hokkaido
    • 年月日
      2016-10-24 – 2016-10-26
  • [学会発表] Effect of microtubule concentration and depletion force on the emergence of collective motion of microtubules2016

    • 著者名/発表者名
      Ai Saito, Ryuhei Suzuki, Tamanna Ishrat Farhana, Arif Md. Rashedul Kabir, Kazuki Sada, Akira Kakugo
    • 学会等名
      The 6th Soft Matter Symposium
    • 発表場所
      Hokkaido University, Sapporo, Hokkaido
    • 年月日
      2016-10-24 – 2016-10-26
  • [学会発表] Effect of Mechanical Deformation of Microtubules on Motor Protein Based Cargo Transportation2016

    • 著者名/発表者名
      Tanjina Afrin, Daisuke Inoue, Arif Md. Rashedul Kabir, Kazuki Sada, Akira Kakugo
    • 学会等名
      The 6th Soft Matter Symposium
    • 発表場所
      Hokkaido University, Sapporo, Hokkaido
    • 年月日
      2016-10-24 – 2016-10-26
  • [学会発表] Buckling of microtubules on a 2D elastic medium2016

    • 著者名/発表者名
      Arif Md. Rashedul Kabir, Daisuke Inoue, Kazuki Sada, Akira Kakugo
    • 学会等名
      The 67th Divisional Meeting on Colloid and Interface Chemistry
    • 発表場所
      Hokkaido University of Education, Asahikawa, Hokkaido
    • 年月日
      2016-09-22 – 2016-09-24
    • 国際学会
  • [学会発表] Controlling the direction of motion of nanoshuttles through photoregulation of their self-organization2016

    • 著者名/発表者名
      Ryuhei Suzuki, Kyohei Uenishi, Jakia Jannat Keya, Arif Md. Rashedul Kabir, Daisuke Inoue, Kazuki Sada, Akinori Kuzuya, Hiroyuki Asanuma, Akira Kakugo
    • 学会等名
      The 67th Divisional Meeting on Colloid and Interface Chemistry
    • 発表場所
      Hokkaido University of Education, Asahikawa, Hokkaido
    • 年月日
      2016-09-22 – 2016-09-24
    • 国際学会
  • [学会発表] Development of a Self-Propelled Motor by Utilizing Oscillatory Beating of Artificial Cilia2016

    • 著者名/発表者名
      R. Sasaki, S. Wada, D. Inoue, A. M. R. Kabir, K. Sada, A. Kakugo
    • 学会等名
      The 67th Divisional Meeting on Colloid and Interface Chemistry
    • 発表場所
      Hokkaido University of Education, Asahikawa, Hokkaido
    • 年月日
      2016-09-22 – 2016-09-24
    • 国際学会
  • [学会発表] Mechanical response of microtubules and modulation of its biochemical functionality2016

    • 著者名/発表者名
      Akira Kakugo, Tanjina Afrin, Arif Md. Rashedul Kabir, Kazuki Sada
    • 学会等名
      65th Annual meeting of The Japan Society for Analytical Chemistry
    • 発表場所
      Hokkaido University, Sapporo, Hokkaido
    • 年月日
      2016-09-15
    • 招待講演
  • [学会発表] Compression Stress Induced Buckling of Microtubule and It’s Effect on Kinesin-based Cargo Transportation in vitro2016

    • 著者名/発表者名
      Akira Kakugo, Arif Md. Rashedul Kabir, Tanjina Afrin, Daisuke Inoue, Kazuki Sada
    • 学会等名
      Biophysical Society Thematic Meeting-Engineering Approaches to Biomolecular Motors: From in vitro to in vivo
    • 発表場所
      Vancouver, CANADA
    • 年月日
      2016-06-14 – 2016-06-17
    • 国際学会

URL: 

公開日: 2018-01-16  

サービス概要 検索マニュアル よくある質問 お知らせ 利用規程 科研費による研究の帰属

Powered by NII kakenhi