| 研究課題/領域番号 |
16K16639
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| 研究機関 | 筑波大学 |
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研究代表者 |
Wang Zhiqiang 筑波大学, 国際統合睡眠医科学研究機構, 研究員 (00762189)
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| 研究期間 (年度) |
2016-04-01 – 2018-03-31
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| キーワード | phosphoproteome / sleep deprivation / Sleepy / sleep need / synaptic proteins / NOS1 |
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| 研究実績の概要 |
In last year’s study, we performed quantitative phosphoproteomic studies of whole mouse brains from two models of sleep/wake perturbation. A combined proteome and phosphoproteome data for 9,410 mouse proteins and 4, 5000 phosphopeptides were examined. Analysis of ad-lib slept, sleep-deprived, and recovery slept mice revealed that sleep and waking drove hypo- and hyper-phosphorylation of brain proteome. In Sleepy mice, a gain-of-function mutation of SIK3 caused constitutively higher sleep need and phosphoproteome imbalance mimicking sleep-deprived condition. The intracerebroventricular (i.c.v.) injection-based and AAV overexpression-based EEG/EMG sleep recording system have been established, the Nos1 inhibitor treatment and overexpress Nos1 on Sleepy are ongoing.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
We have finished phosphoproteomic studies of whole mouse brains from two models of sleep/wake perturbation. The Bioinformatics analysis of the proteome and phosphoproteome data almost completed and generated several new insights. And we found more interesting Sleepy substrates. Two sleep study system, i.c.v. and AAV overexpression, have been established to test the functions of those substrates.
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| 今後の研究の推進方策 |
In new year, we will investigate the function of Sleepy substrates, such as NOS1 through pharmacological and AAV overexpression approaches. Moreover, a manuscript for bioinformatics analysis of the proteome and phosphoproteome data will be prepared.
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| 次年度使用額が生じた理由 |
A new experiment to test new AAV virus system will be done in FY2017.
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| 次年度使用額の使用計画 |
This money will be used to buy new type AAV virus plasmids and packaging reagents like PEI.
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