| 研究実績の概要 |
We successfully published two works that are related to the current project. The first paper deals with the dynamics of the reversible dynamics of the hydrogel and its regulatory behavior to cells (Hoerning et al, Scientific Reports, 7, 7760, 2017). The supramolecular hydrogel is cross-linked by host-guest interactions between β-cyclodextrin (βCD) and adamantane, and were designed for the dynamic regulation of cell-substrate interactions. The magnitude of softening and stiffening of the substrate can be modulated by varying the concentrations of free, competing host molecules (βCD) in solutions. By changing the substrate elasticity at a desired time point, it is possible to switch the micromechanical environments of cells. Here, we use C2C12 muscle cells as a simple biological system to test the dynamics and response dynamics of the hydrogel to the cells. The second paper deals with cardiac dynamics. In particular we developed a novel method to extract and quantify the dynamics of line defects (Hoerning et al, Scientific Reports, 7, 7757, 2017). Line defects spatial separate electrophysiological oscillations in the tissue and are known to be a precursor of deceased states in heart, thus having potential to induce life-threatening fibrillation. In this study we show that line defects can translate, merge, collapse and form stable singularities with even and odd parity while maintaining a stable oscillation of the spiral wave in the tissue.
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