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2018 年度 実績報告書

A new concept of salt handling by Na-binding proteins that immobilize excess Na+ to ease salt stress of seawater teleost fish

研究課題

研究課題/領域番号 16K18575
研究機関東京大学

研究代表者

黄 國成  東京大学, 大気海洋研究所, 特任助教 (40526901)

研究期間 (年度) 2016-04-01 – 2019-03-31
キーワードNa-binding protein / osmoregulation / hypertension / protein purification
研究実績の概要

In this final year, I continued to identify the Na-binding molecules from eel mucus and have purified some Na-binding fractions using various chromatography methods. The fractions showed that the Na-binding molecules are small in molecular size and relatively hydrophilic. I used MS/MS to analyze the protein/peptide composition in fraction but no consistent peptides could be identified. The Na-bindning molecules could be carbohydrate-base molecules where current database is limited to characterize them directly. I characterized different types of mucus cells with novel stainings and markers, which showed that eel produces different types of mucus that are immiscible to form different layers. The compartmentation in mucus is important to the Na-binding phenomenon as the bound Na can be isolated in a separate compartment. This compartmentation could be also important as bound Na is found in separate compartment in human muscle and skin, which correlate with hypertension.
I continued to investigate the molecules after the grant period, and to conrtribute to the hypertension treatment in human.
I presented the results in domestic and international conferences and prepared original manuscripts.

  • 研究成果

    (3件)

すべて 2018 その他

すべて 国際共同研究 (1件) 学会発表 (2件) (うち国際学会 1件)

  • [国際共同研究] University of Hawaii(米国)

    • 国名
      米国
    • 外国機関名
      University of Hawaii
  • [学会発表] An altrnative osmoregulatory strategy by sodium-binding molecules in fish2018

    • 著者名/発表者名
      Marty KS Wong, Nobuhiro Ogawa, Takehiro Tsukada, Yoshio Takei
    • 学会等名
      13th International Congress on the Biology of Fish
    • 国際学会
  • [学会発表] An alternative osmoregulatory strategy by Na-binding molecules in fish2018

    • 著者名/発表者名
      Marty KS Wong, Nobuhiro Ogawa, Takehiro Tsukada, Yoshio Takei
    • 学会等名
      東京大学生命科学シンポジウム| BIO UT

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公開日: 2019-12-27  

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