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2016 年度 実施状況報告書

Beneficial role of plasmalogens in the brain which are reduced by ageing, stress and neuroinflammation

研究課題

研究課題/領域番号 16K19007
研究機関九州大学

研究代表者

Hossain Shamim  九州大学, 医学研究院, 准教授 (40647707)

研究期間 (年度) 2016-04-01 – 2018-03-31
キーワードplasmalogens / hippocampus / neurotrophic factor / spatial learning / orphan GPR / NF-kB / c-Myc
研究実績の概要

In the first year, I could find out that the Plasmalogens (Pls) diet in the mice increased the hippocampal dependent memory. This memory improvement was associated with the increased expression of hippocampal BDNF expression and enhanced localization of TrkB receptors into the lipid rafts. I also found that the Pls diet reduces the amyloid beta (Aβ) protein deposition in the triple transgenic Alzheimer’s disease (AD) mice model. These results have been summarized in a paper and submitted to the journal of Nature Communication. I have further found that Pls enhance the ERK signaling through the G protein-coupled receptors (GPR1, GPR19, GPR21, GPR27 and GPR61). These data have already been published last year (Hossain MS et al., Plos One, e0150846, 2016). In addition, I have also published another paper in the Journal of Neuroscience recently (Hossain MS et al., J Neurosci. 37(15): 4074-4092, 2017.) describing our findings that the reduction of cellular Pls levels in the glial cells lead to the inflammation responses. In this study, I have explained in detail of how the GNPAT expression is reduced by inflammation, ageing and stress responses through the NF-kB and c-Myc. To the establishment of CRISPR-mediated knockout mice of the GPR19 and GPR61, I did not get any positive mice line. This could be due to the embryonic lethal effect of the knockout of these GPCRs. Further experiments are going on to demonstrate the possible interaction of Pls with the GPCRs and their biological role in the brain.

現在までの達成度 (区分)
現在までの達成度 (区分)

2: おおむね順調に進展している

理由

To the establishment of CRISPR-mediated knockout mice of the GPR19 and GPR61, I did not get any positive mice line which was confirmed by genotyping and western blotting assays using tail tissue samples. This could be due to the embryonic lethal effect of the knockout of these GPCRs.

今後の研究の推進方策

There will be no big changes in this project.
For the time being, I will do the best to establish the knockout mice of the orphan GPR 19 and 61.

次年度使用額が生じた理由

Because of unexpected attendance in the international meeting, which was invited as a keynote speaker, the amount of travel expense became over to the planned amount of money. Then I tried to save money for reagents. As a result, small amount of money was even left and should be used next year.

次年度使用額の使用計画

I will use the left money from this year, because the next year is the last of this project.

  • 研究成果

    (9件)

すべて 2017 2016

すべて 雑誌論文 (5件) (うち査読あり 5件、 オープンアクセス 4件、 謝辞記載あり 3件) 学会発表 (4件) (うち国際学会 2件、 招待講演 1件)

  • [雑誌論文] Glucose can epigenetically alter the gene expression of neurotrophic factors in the murine brain cells.2017

    • 著者名/発表者名
      Hossain MS, Oomura Y, Katafuchi, T.
    • 雑誌名

      Molecular Neurobiology

      巻: 印刷中 ページ: 印刷中

    • 査読あり / オープンアクセス / 謝辞記載あり
  • [雑誌論文] Reduction of ether-type glycerophospholipids, plasmalogens, by NF-B signal leading to microglial activation.2017

    • 著者名/発表者名
      Hossain MS, Abe Y, Ali F, Youssef M, Honsho M, Fujiki Y, Katafuchi, T.
    • 雑誌名

      Journal of Neuroscience

      巻: 37 (15) ページ: 4074-4092

    • DOI

      10.1523/JNEUROSCI.3941-15.2017

    • 査読あり / オープンアクセス / 謝辞記載あり
  • [雑誌論文] Neuronal orphan G-protein coupled receptor proteins mediate plasmalogens-induced activation of ERK and Akt signaling.2016

    • 著者名/発表者名
      Hossain MS, Mineno K, Katafuchi T.
    • 雑誌名

      PLoS ONE

      巻: 11(3) ページ: e0150846

    • DOI

      110.1371/journal.pone.0150846

    • 査読あり / オープンアクセス / 謝辞記載あり
  • [雑誌論文] Roles of brain lipids in glial activation.2016

    • 著者名/発表者名
      Hossain MS, Katafuchi T.
    • 雑誌名

      Advances in Neuroimmune Biology

      巻: 6(2) ページ: 61-67

    • 査読あり / オープンアクセス
  • [雑誌論文] Functional involvement of brain lipids in the neuronal survival and anti-neuroinflammation.2016

    • 著者名/発表者名
      Hossain MS, Katafuchi T
    • 雑誌名

      Journal of Fatigue Science

      巻: 11 (2) ページ: 7-17

    • 査読あり
  • [学会発表] Plasmalogens synthesis in the brain is down regulated during neuroinflammation.2017

    • 著者名/発表者名
      Hossain MS, Ali F, Katafuchi T.
    • 学会等名
      第94回日本生理学会大会
    • 発表場所
      アクトシティ浜松(浜松)
    • 年月日
      2017-03-28 – 2017-03-30
  • [学会発表] Plasmalogens improve hippocampal dependent memory by activating BDNF-TrKB signaling in the hippocampus.2016

    • 著者名/発表者名
      Hossain MS, Katafuchi T.
    • 学会等名
      The 8th International Conference on Research in Science and Technology (ICRST).
    • 発表場所
      Flora Grand Hotel (ドバイ United Arab Emirates (UAE)
    • 年月日
      2016-12-20 – 2016-12-21
    • 国際学会 / 招待講演
  • [学会発表] Reduction of Plasmalogens in the brain is associated with microglial activation, stress and aging.2016

    • 著者名/発表者名
      Hossain MS, Abe Y, Ali F, Youssef M, Honsho M, Fujiki Y, Katafuchi T.
    • 学会等名
      The 1st International Plasmalogen Symposium.
    • 発表場所
      九州大学百年講堂(福岡)
    • 年月日
      2016-11-07 – 2016-11-08
    • 国際学会
  • [学会発表] The special lipids, plasmalogens, improve memory by accelerating BDNF-TrkB signaling in the hippocampus .2016

    • 著者名/発表者名
      Hossain MS, Ifuku M, Abe Y, Honsho M, Kawamura J, Miyake K, Fujiki Y, Katafuchi T.
    • 学会等名
      The 39th Annual Meeting of the Japan Neuroscience Society.
    • 発表場所
      パシフィコ横浜(横浜)
    • 年月日
      2016-07-20 – 2016-07-22

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公開日: 2018-01-16  

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