Stimulating the host innate immune response to fight the early stages of malaria infection

2017 年度 実績報告書

• 研究課題/領域番号: 16K21233
• 研究機関: 長崎大学
• 研究代表者: カレトン リチャード 長崎大学, 熱帯医学研究所, 准教授 (10503782)
• 研究期間 (年度): 2016-04-01 – 2018-03-31
• キーワード: Malaria / Pam2Cys / vaccine / antimalarial drug

研究実績の概要

During the last year we analysed how Pam2Cys changes the immune system to protect against malaria parasites in the livers of mice. We found that:
1) Pam2Cys increased blood levels of cytokines, small proteins that effect other cells. It is possible that increased levels of cytokines are involved in killing malaria parasites in the liver.
2) Pam2Cys increased the numbers of Natural Killer (NK) T-cells in the livers of mice. These NK T-cells may be activated by cytokines.
3) Pam2Cys protects the liver against malaria parasite growth at concentrations of 5 nanomoles.
While more work needs to be done to explain exactly how Pam2Cys changes the immune system, our research shows that Pam2Cys both kills parasites and prevents infection in the livers of mice.

研究成果

• [雑誌論文] Schistosoma mansoni infection suppresses the growth of Plasmodium yoelii parasites in the liver and reduces gametocyte infectivity to mosquitoes (2018)
  • 著者名/発表者名: Moriyasu T、Nakamura R、Deloer S、Senba M、Kubo M、Inoue M、Culleton R、Hamano S
  • 雑誌名: PLOS Neglected Tropical Diseases 巻: 12 ページ: e0006197
  • DOI: 10.1371/journal.pntd.0006197
  • 査読あり / オープンアクセス / 国際共著
• [雑誌論文] An assay for the identification of Plasmodium simium infection for diagnosis of zoonotic malaria in the Brazilian Atlantic Forest (2018)
  • 著者名/発表者名: Alvarenga DA, Culleton R, Pina-Costa Ad, Rodrigues D, Junior CB, Silva S, Nunes AJ, J Souza jr JCd, Hirano Z, Moreira ZB, Pissinatti A, Abreu F, Areas AL, Lourenco-de-Oliveira R, Zalis MG, Brasil P, Ferreira-da-Cruz MdF, Daniel-Ribeiro CT, Alves de Brito CF
  • 雑誌名: Scientific Reports 巻: 8 ページ: e18216
  • DOI: 10.1038/s41598-017-18216-x
  • 査読あり / オープンアクセス / 国際共著
• [雑誌論文] Outbreak of human malaria caused by Plasmodium simium in the Atlantic Forest in Rio de Janeiro: a molecular epidemiological investigation (2017)
  • 著者名/発表者名: Brasil P, Zalis M G, de Pina-Costa A, Siqueira AM, Bianco Junior C, Silva S, Areas ALL, Pelajo-Machado M, de Alvarenga DAM, Santelli ACFdS, Albuquerque HG, Cravo P, de Abreu FVS, Peterka CL, Zanini GM, Suarez-Mutis MC, Pissinatti A, Lourenco-de-Oliveira R, Brito CFAd, Ferreira-da-Cruz MdF, Culleton R, Daniel-Ribeiro CT
  • 雑誌名: The Lancet Global Health 巻: 5 ページ: e1038～e1046
  • DOI: 10.1016/s2214-109x(17)30333-9
  • 査読あり / オープンアクセス / 国際共著
• [雑誌論文] Return of chloroquine sensitivity to Africa? Surveillance of African Plasmodium falciparum chloroquine resistance through malaria imported to China (2017)
  • 著者名/発表者名: Lu F、Zhang M、Culleton RL、Xu S、Tang J、Zhou H、Zhu G、Gu Y、Zhang C、Liu Y、Wang W、Cao Y、Li J、He X、Cao J、Gao Q
  • 雑誌名: Parasites & Vectors 巻: 10 ページ: 355～355
  • DOI: 10.1186/s13071-017-2298-y
  • 査読あり / オープンアクセス / 国際共著
• [雑誌論文] Artemisinin-Resistant Plasmodium falciparum in Africa (2017)
  • 著者名/発表者名: Lu F, Culleton R, Cao J
  • 雑誌名: New England Journal of Medicine 巻: 377 ページ: 305～306
  • DOI: 10.1056/nejmc1705789
  • 査読あり / オープンアクセス / 国際共著
• [雑誌論文] Rapid identification of genes controlling virulence and immunity in malaria parasites (2017)
  • 著者名/発表者名: Abkallo H, Martinelli A, Inoue M, Ramaprasad A, Xangsayarath P, Gitaka J, Tang J, Yahata K, Zoungrana A, Mitaka H, Hunt P, Carter R, Kaneko O, Mustonen V, Illingworth CJR, Pain A, Culleton R
  • 雑誌名: PLOS Pathogens 巻: 13 ページ: e1006447
  • DOI: 10.1371/journal.ppat.1006447
  • 査読あり / オープンアクセス / 国際共著
• [雑誌論文] Detection of the Plasmodium falciparum Kelch-13 gene P553L mutation in sporozoites isolated from mosquito salivary glands in South-Central Vietnam (2017)
  • 著者名/発表者名: Maeno Y、Quang NT、Culleton R、Kawai S、Masuda G、Hori K、Nakazawa S、Marchand RP
  • 雑誌名: Parasites & Vectors 巻: 10 ページ: 308
  • DOI: 10.1186/s13071-017-2247-9
  • 査読あり / オープンアクセス / 国際共著
• [学会発表] Phenotypic, Genotypic, and Transcriptomic Characterisation of the Plasmodium vinckei clade of Rodent Malaria Parasites (2017)
  • 著者名/発表者名: Richard Culleton
  • 学会等名: 第１６回あわじしま感染症・免疫フォーラム
• [学会発表] Genetic Analysis of Drug Resistance Genes in Plasmodium falciparum, Plasmodium malariae andPlasmodium ovale Isolated from Asymptomatic individuals in South-East Nigeria (2017)
  • 著者名/発表者名: Ernest Medard（研究協力者）
  • 学会等名: 第16回あわじしま感染症・免疫フォーラム