| 研究課題/領域番号 |
16K21272
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| 研究機関 | 横浜市立大学 |
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研究代表者 |
Goulas Spyros 横浜市立大学, 医学研究科, 特任助教 (90644352)
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| 研究期間 (年度) |
2016-04-01 – 2018-03-31
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| キーワード | Epithelial Homeostasis / Overproliferation / Cell Death / Barrier Malfunction |
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| 研究実績の概要 |
Throughout the lifespan of an organism, there is constant cellular turnover, known as homeostasis. When perturbed, this can lead to overproliferation or premature cell death, causing diseases such as cancer. Epithelia have some of the highest levels of cellular turnover and are also highly susceptible to cancers. Loss of polarity has been associated with cancer, but how remains unclear. To understand how epithelia maintain homeostasis, in FY2016 I have screened knockdown lines using a candidate approach of known cell polarity regulators in MDCK cells. I have identified and verified several candidates that resulted in phenotypes during epithelial homeostasis. Some of the phenotypes observed included overproliferation, excessive cell death and epithelial barrier malfunction. These candidates are now being further characterized.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
3: やや遅れている
理由
Initially, due to technical difficulties of establishing a live automated imaging-based screening system, some delays occurred. These issues could not be resolved so I opted for a ‘non-live’ alternative strategy using stable knockdown to analyze defects in epithelial homeostasis. This circumvented many of the issues and now things are running smoothly.
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| 今後の研究の推進方策 |
In FY2017, I plan to continue with the proposed project with some alterations. Based on the distinct phenotypes observed, it is possible to categorize them into groups and focus on identifying the potential downstream molecular targets. Furthermore, to establish whether targets identified in vitro have a physiological function in vivo, I will be using the developing Drosophila adult dorsal epithelium (notum).
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| 次年度使用額が生じた理由 |
In the next fiscal year FY2017, I plan to buy/make antibodies, including Drosophila specific ones, which was originally planned for FY2016. Furthermore, as I was unable to promote my research at an international conference in FY2016, I will do this in FY2017. In FY2017, I also plan for travel visits to my research collaborators at Akita University.
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| 次年度使用額の使用計画 |
671,440 円 for Article Costs. 400,000 円 to attend an International Research Conference to present the current research. 400,000 円 for Travel Costs to visit collaborators.
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