高等植物のジェネティックスとエピジェネティックス

2008 年度 研究成果報告書

• 研究課題/領域番号: 17207002
• 研究種目: 基盤研究(A)
• 配分区分: 補助金
• 応募区分: 一般
• 研究分野: 遺伝・ゲノム動態
• 研究機関: 基礎生物学研究所
• 研究代表者: 飯田 滋 基礎生物学研究所, 分子遺伝学研究部門, 教授 (30012777)
• 研究分担者: 寺田 理枝 基礎生物学研究所, 分子遺伝学研究部門, 助教 (30137799) ; 星野 敦 基礎生物学研究所, 分子遺伝学研究部門, 助教 (80312205) ; 栂根 一夫 基礎生物学研究所, 分子遺伝学研究部門, 助教 (50343744)
• 研究期間 (年度): 2005 – 2008
• キーワード: 遺伝学 / 遺伝子 / バイオテクノロジー / ゲノム / 植物

研究概要

江戸時代に我国で花卉園芸化されて多数の花の色や形態に関する自然突然変異体が分離され、戦前に世界をリードし、我国の文化遺産でもあるアサガオの古典遺伝学的成果の内で、花の色や模様に的を絞り、分子遺伝学的観点から変異の実態を解明し、ジェネティク及びエピジェネティクな発現制御機構の解明を行なった。また、ゲノム配列が解析されてモデル植物となったイネを用いて、我々が独自に開発した変異創成法である相同組換えによる遺伝子ターゲティング法による変異体作出と自然条件下で転移を制御できるDNA型トランスポゾンの転移制御機構の解析も行なった。

研究成果

• [雑誌論文] Characterization of autonomous Dart1 transposons belonging to the hAT superfamily in rice. (2008)
  • 著者名/発表者名: Z. Shimatani, K. Takagi, C.H. Eun, M. Maekawa, H. Takahara, A. Hoshino, Q. Qian, R. Terada, Y. Johzuka-Hisatomi, S. lida and K. Tsugane
  • 雑誌名: Mol. Gen. Genomics 281 ページ: 329-344
  • 査読あり
• [雑誌論文] Identification of r mutations conferring white flowers in the Japanese morning glory (2008)
  • 著者名/発表者名: A. Hoshino, K.I. Park and S. lida
  • 雑誌名: Ipomoea nil. J. Plant Res. 122 ページ: 15-222
  • 査読あり
• [雑誌論文] Efficient transfer of base changes from a vector to the rice genome by homologous recombination : involvement of heteroduplex formation and mismatch correction. (2008)
  • 著者名/発表者名: Y. Johzuka-Hisatomi, R. Terada and S. lida
  • 雑誌名: Nucleic Acids Res. 36 ページ: 4727-4735
  • 査読あり
• [雑誌論文] Alternative splicing of the rice OsMET1 genes encoding maintenance DNA methyltransferase. (2008)
  • 著者名/発表者名: T. Yamauchi, S. Moritoh, Y. Johzuka-Hisatomi, A. Ono, R. Terada, I. Nakamura and S. lida
  • 雑誌名: J. Plant Physiol 165 ページ: 1774-1782
  • 査読あり
• [雑誌論文] The gravity-regulated growth of axillary buds is mediated by a mechanism different from decapitation-induced release. (2008)
  • 著者名/発表者名: D. Kitazawa, Y. Miyazawa, N. Fujii, A. Hoshino, S. lida, E. Nitasaka and H. Takahashi
  • 雑誌名: Plant Cell Physiol 49 ページ: 891-900
  • 査読あり
• [雑誌論文] Distribution and mapping of an active autonomous aDart element responsible for mobilizing nonautonomous nDart1 transposons in cultivated rice varieties. (2008)
  • 著者名/発表者名: H. Nishimura, N. Ahmed, K. Tsugane, S. lida, and M. Maekawa
  • 雑誌名: Theor. Appl. Genet. 116 ページ: 395-405
  • 査読あり
• [雑誌論文] Gene targeting by homologous recombination as a biotechnological tool for rice functional genomics. (2007)
  • 著者名/発表者名: R. Terada, Y. Johzuka-Hisatomi, M. Saitoh, H. Asao, and S. lida
  • 雑誌名: Plant Physiol 144 ページ: 846-856
  • 査読あり
• [雑誌論文] Transposon display for active DNA transposons in rice. (2007)
  • 著者名/発表者名: K. Takagi, N. lshikawa, M. Maekawa, K. Tsugane, and S. lida
  • 雑誌名: Genes Genet. Syst. 82 ページ: 109-122
  • 査読あり
• [雑誌論文] Spontaneous mutations caused by an active Helitron transposon, Hel-It1, in morning glory, Ipomoea tricolor. (2007)
  • 著者名/発表者名: J.D. Choi, A. Hoshino, K.I. Park, I.S. Park, and S. lida
  • 雑誌名: Plant J. 49 ページ: 924-934
  • 査読あり
• [雑誌論文] A bHLH regulatory gene in the common morning glory, Ipomoea purpurea, controls anthocyanin biosynthesis in flowers, proanthocyanidin and phytomelanin pigmentation in seeds, and seed trichome formation. (2007)
  • 著者名/発表者名: K.I. Park, N. lshikawa, Y. Morita, J.D. Choi, A. Hoshino, and S. lida
  • 雑誌名: Plant J. 49 ページ: 641-659
  • 査読あり
• [雑誌論文] The Rc and Rd genes are involved in proanthocyanidin synthesis in the rice pericarp. (2007)
  • 著者名/発表者名: T. Furukawa, M. Maekawa, T. Oki, I. Suda, S. lida, H. Shimada, I. Takamure and K. Kadowaki
  • 雑誌名: Plant J. 49 ページ: 91-102
  • 査読あり
• [雑誌論文] An active DNA transposon nDart causing leaf variegation and mutable dwarfism and its related elements in rice. (2006)
  • 著者名/発表者名: K. Tsugane, M. Maekawa, K. Takagi, H. Takahara, Q, Qian, C.H, Eun, and S. lida
  • 雑誌名: Plant J. 45 ページ: 46-57
  • 査読あり
• [雑誌論文] Isolation of cDNAs for R2R3-MYB, bHLH, and WDR transcriptional regulators and identification of c and ca mutations conferring white flowers in the Japanese morning glory. (2006)
  • 著者名/発表者名: Y. Morita, M. Saitoh, A. Hoshino, E. Nitasaka, and S. lida
  • 雑誌名: Plant Cell Physiol. 47 ページ: 457-470
  • 査読あり
• [雑誌論文] Modification of endogenous natural genes by gene targeting in rice and other higher plants. (2005)
  • 著者名/発表者名: S. lida, and R. Terada
  • 雑誌名: Plant Mol. Biol. 59 ページ: 205-219
  • 査読あり
• [雑誌論文] Molecular mapping of a fertility restorer gene for Owen cytoplasmic male sterility in sugar beet. (2005)
  • 著者名/発表者名: E. Hagihara, N. Itchoda, Y. Habu, S. lida, T. Mikami, and T. Kubo
  • 雑誌名: Theor. Appl. Genet. 111 ページ: 250-255
  • 査読あり
• [雑誌論文] Japanese morning glory dusky mutants displaying reddish-brown or purplish-grey flowers are deficient in a novel glycosylation enzyme for anthocyanin biosynthesis, UDP-glucose :anthocyan id in 3-O-glucoside-2"-O- glucosyltransferase, due to 4-bp insertions in the gene. (2005)
  • 著者名/発表者名: Y. Morita, A. Hoshino, Y. Kikuchi, H. Okuhara, E. Ono, Y. Tanaka, Y. Fukui, N. Saito, E. Nitasaka, H. Noguchi, and S. lida
  • 雑誌名: Plant J. 42 ページ: 353-363
  • 査読あり
• [雑誌論文] Characterization of a novel Na+/H+ antiporter gene InNHX2 and comparison of InNHX2 with InNHX1, which is responsible for blue flower coloration by increasing the vacuolar pH in the Japanese morning glory. (2005)
  • 著者名/発表者名: M. Ohnishi, S. Fukada-Tanaka, A. Hoshino, J. Takada, Y. Inagaki, and S. lida
  • 雑誌名: Plant Cell Physiol. 46 ページ: 259-267
  • 査読あり
• [雑誌論文] Acylated peonidin glycosides from duskish mutant flowers of Ipomoea nil. (2005)
  • 著者名/発表者名: N. Saito, K. Toki, Y. Morita, A. Hoshino, S. lida, A. Shigihara, and T. Honda
  • 雑誌名: Phytochemistry 66 ページ: 1852-1860
  • 査読あり
• [雑誌論文] Shoot circumnutation and winding movements require gravisensing cells. (2005)
  • 著者名/発表者名: D. Kitazawa, Y. Hatakeda, M. Kamada, N. Fujii, Y. Miyazawa, A. Hoshino, S. lida, H. Fukaki, M. T. Morita, M. Tasaka, H. Suge, and H. Takahashi
  • 雑誌名: Proc. Natl. Acad. Sci. USA 102 ページ: 18742-18747
  • 査読あり
• [学会発表] Efficient Transfer of Base Changes From a Vector to the Genome by Homologous Recombination in Rice Gene Targeting : Involvement of Heteroduplex Formation and Mismatch Correction. (2008)
  • 著者名/発表者名: Johzuka-Hisatomi, Y. and lida, S.
  • 学会等名: 55th NIBB Conference, Frontiers of Plant Science in the 21st Century.
  • 発表場所: Okazaki, Japan
  • 年月日: 20080913-15
• [学会発表] Targeted Disruption of the OsDRMla Gene, a Rice Homolog of DOMAINS REARRANGED METHYLTRANSFERASE, by Homologous Recombination. (2008)
  • 著者名/発表者名: Moritoh, S., Terada, R., Johzuka-Hisatomi, Y., Eun, C., Yamaguchi, K., Ono, A. and lida, S.
  • 学会等名: 55th NIBB Conference, Frontiers of Plant Science in the 21st Century.
  • 発表場所: Okazaki, Japan
  • 年月日: 20080913-15
• [学会発表] Epigenetic Regulation of Transposition Activity of the Dart DNA Transposon System in Rice. (2008)
  • 著者名/発表者名: Tsugane, K., Maekawa, M., Eun, C., Takagi, K., Nishimura, H., Shimatani, Z., Hayashi, M. and lida, S.
  • 学会等名: 55th NIBB Conference, Frontiers of Plant Science in the 21st Century.
  • 発表場所: Okazaki, Japan
  • 年月日: 20080913-15
• [学会発表] Epigenetic regulation of flower pigmentation in the morning glory. (2008)
  • 著者名/発表者名: Hoshino, A., Choi, J.D., Park, K.I. and lids, S.
  • 学会等名: The 55th NIBB Conference and Arabidopsis Workshop 2008, Frontiers of Plant Science in the 21st Century.
  • 発表場所: Okazaki, Japan
  • 年月日: 20080913-15
• [学会発表] Epigenetic regulation of flower pigmentation in the morning glory. (2008)
  • 著者名/発表者名: Hoshino, A., Choi, J.D., Park, K.I. and lids, S.
  • 学会等名: 6th NIBB-EMBL Joint Meeting : Evolution of epigenetic regulation.
  • 発表場所: Heidelberg, Germany
  • 年月日: 20080317-19
• [学会発表] Epigenetic gene regulation in the Japanese morning glory and rice. (2008)
  • 著者名/発表者名: lida, S., Morita, Y., Johzuka-Hisatomi, Y., Yamauchi, T. and Moritoh, S.
  • 学会等名: 6th NIBB-EMBL Joint Meeting : Evolution of epigenetic regulation.
  • 発表場所: Heidelberg, Germany
  • 年月日: 20080317-19
• [学会発表] Endogenous DNA transposon, nDart-tagging system in rice. (2007)
  • 著者名/発表者名: Maekawa, M., Tsugane, K. and lida, S.
  • 学会等名: Workshop on Development Genetics and Molecular Breeding in Rice.
  • 発表場所: Hongzhou, China
  • 年月日: 20071117-19
• [学会発表] Flower coloration and vacuolar alkalization in the Japanese morning glory. (2007)
  • 著者名/発表者名: Hoshino, A., Fukada-Tanaka, S., Inagaki, Y., Yamaguchi, T., Ohnishi, M. and lida, S.
  • 学会等名: 9th World Petunia Days.
  • 発表場所: Amsterdam, The Netherlands
  • 年月日: 20071028-31
• [学会発表] Gene targeting by homologus recombination as a biotechnological tool for rice functional genomics. (2007)
  • 著者名/発表者名: Johzuka-Hisatomi, Y., Terada, R., Nakazono, M. and lida, S.
  • 学会等名: 5th International Symposium of Rice Functional Genomics.
  • 発表場所: Tsukuba, Japan
  • 年月日: 20071015-17
• [学会発表] Gene targeting by homologous recombination in rice. (2007)
  • 著者名/発表者名: Terada, R., Johzuka-Hisatomi, Y., Yamauchi, T. and lida S.
  • 学会等名: 1st EMBO Workshop on Plant DNA Repair and Recombination.
  • 発表場所: Presquile de Giens, France
  • 年月日: 20070531-0603
• [学会発表] Potent nDart-mediated gene tagging system in rice. (2007)
  • 著者名/発表者名: Maekawa, M., Tsugane, K. and lida S.
  • 学会等名: International Symposium on Rice Mutants, Functional Genomics and Bio-technology Breeding.
  • 発表場所: Hongzhou, China
  • 年月日: 20070329-0401
• [学会発表] Gene tagging by activating DNA transposon nDart in indica rice. (2007)
  • 著者名/発表者名: Ahmed, N., Maekawa, M., Takahara, H., Takagi, K., Tsuqane, K. and lida S.
  • 学会等名: 2nd International Conference of Plant Molecular Breeding.
  • 発表場所: Sanya, China
  • 年月日: 20070323-27
• [学会発表] Epigenetic regulation of an active DNA transposon nDart under natural growth condition, and its application for gene tagging in rice. (2007)
  • 著者名/発表者名: Tsugane, K., Maekawa, M., Takagi, K., Eun, C. and lida S.
  • 学会等名: The sixth Cold Spring Harbor winter conference PLANT GENOMES.
  • 発表場所: Cold Spring Harbor, USA
  • 年月日: 20070315-20
• [学会発表] Generation of rice DDM1 knock-out mutants by homologous recombination-promoted gene targeting. (2007)
  • 著者名/発表者名: Terada, R., Johzuka-Hisatomi, Y. and lida S.
  • 学会等名: UCR's 24th Symposium in Plant Biology "Gene silencing : the biology of small RNAs and the epigenome".
  • 発表場所: Riverside, USA
  • 年月日: 20070118-20
• [学会発表] Post-transcriptional gene silencing is involved in the formation of characteristic pigmentation patterns in flowers of the Japanese morning glory. (2007)
  • 著者名/発表者名: Morita, Y., Hoshino, A. and lida S.
  • 学会等名: 24th Symposium in Plant Biology, Riverside.
  • 発表場所: Riverside, USA
  • 年月日: 20070118-20
• [学会発表] Identification of an active DNA transposon nDart under natural growth condition, and its application for gene tagging in rice. (2006)
  • 著者名/発表者名: Tsugane, K., Maekawa, M., Takagi, K., Eun, C. and lida, S.
  • 学会等名: The 5th Plant Genomics European Meeting
  • 発表場所: Venice, Italy
  • 年月日: 20061011-14
• [学会発表] Gene targeting by homologous recombination in rice. (2006)
  • 著者名/発表者名: Terada, R., Johzuka-Hisatomi, Y., Saitoh, M. and lid S.
  • 学会等名: The 8th International Congress of Plant Molecular Biology.
  • 発表場所: Adelaide, Australia
  • 年月日: 20060820-25
• [学会発表] Flower pigmentation patterns and gene silencing in the Japanese morning glory. (2006)
  • 著者名/発表者名: Hoshino, A., Morita, Y., Park, K.I., Ohnishi, M. and lida, S.
  • 学会等名: 2006 Keystone Symposia, RNAi and Related Pathways.
  • 発表場所: British Columbia, Canada
  • 年月日: 20060126-31
• [学会発表] A transposon display for nonautonomous DNA-based active rice transposons, nDarts. (2005)
  • 著者名/発表者名: Takagi, K., Maekawa, M., Tsugane, K. and lida, S.
  • 学会等名: 5th International Rice Genetics Symposium and 3rd International Rice Functional Genomics Symposium.
  • 発表場所: Manila, Philippines
  • 年月日: 20051119-23
• [学会発表] Nonautonomous DNA-based active rice transposons nDarts and their application to gene tagging. (2005)
  • 著者名/発表者名: Maekawa, M., Takahara, H., Qian, Q., Tsugane, K. and lida, S.
  • 学会等名: 5th International Rice Genetics Symposiumand 3rd International Rice Functional Genomics Symposium.
  • 発表場所: Manila, Philippines
  • 年月日: 20051119-23
• [学会発表] The modification of endogenous genes by homologous recombination in rice. (2005)
  • 著者名/発表者名: lida, S., Johzuka-Hisatomi, Y., Saitoh, M. and Terada, R.
  • 学会等名: 5th International Rice Genetics Symposium 3rd International Rice Functional Genomics Symposium.
  • 発表場所: Manila, Philippines
  • 年月日: 20051119-23
• [図書] Homologous recombination-dependent gene targeting and an active DNA transposon nDart-promoted gene tagging for rice functional genomics.(H. Hirano, A. Hirai, Y. Sano, and T. Sasaki eds., Rice Biology in the Genomic Era)(81-94) (2008)
  • 著者名/発表者名: Y. Johzuka-Hisatomi, M. Maekawa, K. Takagi, C.H. Eun, T. Yamauchi, Z. Shimatani, N. Ahmed, H. Urawa, K. Tsuciane, R. Terada and S. lida
  • 出版者: Biotechnology in Agriculture and Forestry
• [図書] Gene targeting by homologous recombination for rice functional genomics.(N.M. Upadhyaya ed. Rice Functional enomics-Challenges, Progress and Prospects)(pp.273-289) (2007)
  • 著者名/発表者名: S. lida, Y. Johzuka-Hisatomi and R. Terada
  • 出版者: Springer, Berlin, Heidelberg, New York
• [図書] Flavonoid pigments as tools in molecular genetics(E. Glotewold ed. The Science of Flavonoids)(pp.147-173) (2006)
  • 著者名/発表者名: S. Chopra, A. Hoshino, J. Boddu, and S. lida
  • 出版者: Springer, Berlin, Heidelberg, New York