アジアにおけるエキノコックス、テニア条虫の種分化、病原性分化、分子進化調査研究

2008 年度 研究成果報告書

• 研究課題/領域番号: 17256002
• 研究種目: 基盤研究(A)
• 配分区分: 補助金
• 応募区分: 海外学術
• 研究分野: 寄生虫学(含衛生動物学)
• 研究機関: 旭川医科大学
• 研究代表者: 伊藤 亮 旭川医科大学, 医学部, 教授 (70054020)
• 研究分担者: 中尾 稔 旭川医科大学, 医学部, 准教授 (70155670) ; 中谷 和宏 旭川医科大学, 医学部, 准教授 (70109388) ; 迫 康仁 旭川医科大学, 医学部, 助教 (40312459) ; 柳田 哲矢 旭川医科大学, 医学部, 助教 (40431837) ; 岡本 宗裕 鳥取大学, 農学部, 准教授 (70177096)
• 研究期間 (年度): 2005 – 2008
• キーワード: 地球規模寄生虫感染症 / 人獣共通寄生虫疾患 / エキノコックス症 / 脳嚢虫症

研究概要

エキノコックス症、脳嚢虫症は新興・再興寄生虫感染症として国際的な問題になっている。本研究ではアジアにおけるこれらの寄生虫の多様性(種、病原性)ならびに地理拡散について、進化学的な観点から調査研究を展開する。条虫の分類学的再検討を含め、種間変異、種内変異の解析を中心に、それぞれの種の多様性の解析を試み、(1)遺伝子ならびに形態学的解析に基づく分類学的再検討、(2)病原性の多様性、(3)条虫症の地球上での分布拡散、(4)進化学的考察についての解析・考察を試みる。

研究成果

• [雑誌論文] Kern P. Close relationship between clinical regression and specific serology in the follow-up of patients with alveolar echinococcosis in different clinical stages (2009)
  • 著者名/発表者名: Tappe D, Frosch M, Sako Y, Itoh S, Gruner B, Reuter S, Nakao M, Ito A
  • 雑誌名: American Journal of Tropical Medicine and Hygiene 80 ページ: 792-797
  • 査読あり
• [雑誌論文] Schantz PM, Cavero C, Cabrera L. Molecular identification of Echinococcus isolates from Peru (2009)
  • 著者名/発表者名: Moro PL, Nakao M, Ito A
  • 雑誌名: Parasitology International 58 ページ: 184-186
  • 査読あり
• [雑誌論文] Genetic analysis of Echinococcus multilocularisoriginating from a patient with alveolar echinococcosis occurring in Minnesota in 1977 (2008)
  • 著者名/発表者名: Yamasaki H, Nakao M, Nakaya K, Schantz PM, Ito A
  • 雑誌名: American Journal of Tropical Medicine and Hygiene 79 ページ: 245-247
  • 査読あり
• [雑誌論文] Scheifler R, Craig PS. Small mammal assemblages and habitat distribution in the northern Junggar Basin, Xinjiang China : a pilot survey. (2008)
  • 著者名/発表者名: Giraudoux P, Zhou H, Quere JP, Raoul F, Delattre P, Volobouev V, Deforet T, Ito A, Mamuti W
  • 雑誌名: Mammalia 72 ページ: 309-319
  • 査読あり
• [雑誌論文] Species identification of human echinococcosis using histopathology and genotyping in northwestern China (2008)
  • 著者名/発表者名: Li T, Ito A, Nakaya K, Qiu JM, Nakao M, Zhen R, Xiao N, Chen XW, Giraudoux P, Craig PS
  • 雑誌名: Transactions of the Royal Society of Tropical Medicine and Hygiene 102 ページ: 585-590
  • 査読あり
• [雑誌論文] Qiu J, Craig PS. Evaluation of three PCR assays for the identification of the sheep strain (genotype 1) of Echinococcus granulosus in canid feces and parasite tissues (2008)
  • 著者名/発表者名: Boufana BS, Campos-Ponce M, Naidich A, Buishi I, Lahmar S, Zeyhle E, Jenkins DJ, Combes B, Wen H, Xiao N, Nakao M, Ito A
  • 雑誌名: American Journal of Tropical Medicine and Hygiene 78 ページ: 777-783
  • 査読あり
• [雑誌論文] Echinococcoses and Tibetan communities (2008)
  • 著者名/発表者名: Craig PS, Li T, Qiu J, Zhen R, Wang Q, Giraudoux P, Ito A, Heath D, Warnock B, Schantz P, Yang W
  • 雑誌名: Emerging Infectious Diseases 14 ページ: 1674-1675
  • 査読あり
• [雑誌論文] Molecular and immunological diagnosis of taeniasis and cysticercosis in Asia and the Pacific (2008)
  • 著者名/発表者名: Ito A, Sako Y, Nakao M, Nakaya K, Okamoto M, Wandra T, Kandun IN, Anantaphruti MT, Waikagul J, Li T, Qiu D
  • 雑誌名: Southeast Asian Journal of Tropical Medicine and Public Health 39(Suppl 1) ページ: 37-46
  • 査読あり
• [雑誌論文] Genetic characterization and phylogenetic position of Echinococcus felidis Ortlepp, 1937 (Cestoda : Taeniidae) from the African lion (2008)
  • 著者名/発表者名: Huttner M, Nakao M, Wassermann T, Siefert L, Boomker DF, Dinkel A, Sako Y, Mackenstedt U, Romig T, Ito A
  • 雑誌名: International Journal for Parasitology 38 ページ: 861-868
  • 査読あり
• [雑誌論文] 医学と医療の最前線 エキノコックス症に関する診断法の進展 (2008)
  • 著者名/発表者名: 伊藤亮, 中尾稔, 迫康仁, 中谷和宏, 石川裕司, 柳田哲矢
  • 雑誌名: 日本内科学会雑誌 97 ページ: 1900-1909
  • 査読あり
• [雑誌論文] Molecular characterization of a novel gene encoding an 8-kDa-subunit of antigen B from Echinococcus granulosus genotypes 1 and 6 (2007)
  • 著者名/発表者名: Mamuti W, Sako Y, Bart JM, Nakao M, Ma X, Wen H, Ito A
  • 雑誌名: Parasitology International 56 ページ: 313-316
  • 査読あり
• [雑誌論文] Cloning and characterization of cathepsin L-like peptidases of Echinococcus multilocularis metacestodes (2007)
  • 著者名/発表者名: Sako Y, Yamasaki H, Nakaya K, Nakao M, Ito A
  • 雑誌名: Molecular and Biochemical Parasitology 154 ページ: 81-189
  • 査読あり
• [雑誌論文] A molecular phylogeny of the genus Echinococcus inferred from complete mitochondrial genomes (2007)
  • 著者名/発表者名: Nakao M, McManus DP, Schantz PM, Craig PS, Ito A
  • 雑誌名: Parasitolog 134 ページ: 13-722
  • 査読あり
• [雑誌論文] Echinococcosis : serological detection of patients and molecular identification of parasites (2007)
  • 著者名/発表者名: Ito A, Nakao M, Sako Y
  • 雑誌名: Future Microbiology 2 ページ: 439-449
  • 査読あり
• [雑誌論文] Sympatric occurrence of Taenia solium, T. saginata, and T. asiatica, Thailand (2007)
  • 著者名/発表者名: Anantaphruti MT, Yamasaki H, Nakao M, Waikagul J, Wattanakulpanich D, Nuamtanong S, Maipanich W, Pubampen S, Sanguankiat S, Muennoo C, Nakaya K, Sato MO, Sako Y, Okamoto M, Ito A
  • 雑誌名: Emerging Infectious Diseases 13 ページ: 1413-1416
  • 査読あり
• [雑誌論文] Comparison of several commercial serologic kits and Em18 serology for detection of human alveolar echinococcosis (2007)
  • 著者名/発表者名: Bart JM, Piarroux M, Sako Y, Grenouillet F, Bresson-Hadni S, Piarroux R, Ito A
  • 雑誌名: Diagnostic Microbiology and Infectious Diseases 59 ページ: 93-95
  • 査読あり
• [雑誌論文] Community surveys and risk factor analysis of human alveolar and cystic echinococcosis in Ningxia Hui autonomous region China. (2007)
  • 著者名/発表者名: Yang YR, Sun T, Li Z, Zhang J, Teng J, Liu X, Liu R, Zhao R, Jones MK, Wang Y, Wen H, Feng X, Zhao Q, Zhao Y, Shi D, Bartholomot B, Vuitton DA, Pleydell D, Giraudoux P, Ito A, Danson MF, Boufana B, Craig PS, Williams GM, McManus DP
  • 雑誌名: Bulletin of the World Health Organization 84 ページ: 714-721
  • 査読あり
• [雑誌論文] A correlative study of ultrasound with serology in an area in China co-endemic for human alveolar and cystic echinococcosis (2007)
  • 著者名/発表者名: Yang YR, Craig PS, Ito A, Vuitton DA, Giraudoux P, Sun T, Williams GM, Huang Z, Li Z, Wang Y, Teng J, Li Y, Huang L, Wen H, Jones MK, McManus DP
  • 雑誌名: Tropical Medicine and International Health 12 ページ: 637-646
  • 査読あり
• [雑誌論文] Taeniasis in Mongolia, 2002-2006 (2007)
  • 著者名/発表者名: Myadagsuren N, Davaajav A, Wandra T, Sandar T, Ichikhorloo P, Yamasaki H, Sako Y, Nakao M, Sato MO, Nakaya K, Ito A
  • 雑誌名: American Journal of Tropical Medicine and Hygiene 77 ページ: 342-346
  • 査読あり
• [雑誌論文] Assessment of use of microsatellite polymorphism analysis for improving spatial distribution tracking of Echinococcus multilocularis (2007)
  • 著者名/発表者名: Knapp J, Bart JM, Glowatzki ML, Ito A, Gerard S, Maillard S, Piarroux R, Gottstein B.
  • 雑誌名: Journal of Clinical Microbiology 45 ページ: 2943-2950
  • 査読あり
• [雑誌論文] Mitochondrial genomes of the human broad tapeworms Diphyllobothrium latum and Diphyllobothrium nihonkaiense (Cestoda : Diphyllobothriidae) (2007)
  • 著者名/発表者名: Nakao M, Abmed D, Yamasaki H, Ito A
  • 雑誌名: Parasitology Research 101 ページ: 233-236
  • 査読あり
• [雑誌論文] Taeniasis/cysticercosis in a Tibetan population in Sichuan province (2006)
  • 著者名/発表者名: Li T, Craig PS, Ito A, Chen X, Qiu D, Qiu J, Sato MO, Wandra T, Bradshaw H, Li L, Yang Y, Wang Q
  • 雑誌名: China. Acta Tropica 100 ページ: 223-231
  • 査読あり
• [雑誌論文] Dual infection of animal hosts with different Echinococcus species in the eastern Qinghai-Tibet plateau region of China (2006)
  • 著者名/発表者名: Xiao N, Nakao M, Qiu J, Budke CM, Giraudoux P, Craig PS, Ito A
  • 雑誌名: American Journal of Tropical Medicine and Hygiene 75 ページ: 292-294
  • 査読あり
• [雑誌論文] Evaluation of purified Taenia solium glycoproteins and recombinant antigens in the serologic detection of human and swine cysticercosis (2006)
  • 著者名/発表者名: Sato MO, Sako Y, Nakao M, Yamasaki H, Nakaya K, Ito A
  • 雑誌名: Journal of Infectious Diseases 194 ページ: 1783-1790
  • 査読あり
• [雑誌論文] Geno- typin g of human cystic echinococcosis in Xinjiang (2006)
  • 著者名/発表者名: Bart JM, Abdukader M, Zhang YL, Lin RY,Wang YH, Nakao M, Ito A, Craig PS, Piarroux R, Vuitton DA, Wen H
  • 雑誌名: PR China. Parasitology 133 ページ: 571-579
  • 査読あり
• [雑誌論文] Unique family clustering of human echinococcosis case in a Chinese community (2006)
  • 著者名/発表者名: Yang YR, Elis M, Sun T, Li Z, Liu X, Vuitton DA, Bartholomot B, Giraudoux P, Craig PS, Boufana B, Wang Y, Feng Z, Wen H, Ito A, McManus DP
  • 雑誌名: American Journal of Tropical Medicine and Hygiene 74 ページ: 487-494
  • 査読あり
• [雑誌論文] Towards the international collaboration for detection, surveillance and control of taeniasis/ cysticercosis and echinococcosis in Asia and the Pacific (2006)
  • 著者名/発表者名: Ito A, Wandra T, Sato MO, Mamuti W, Xiao N, Sako Y, Nakao M, Yamasaki H, Nakaya K, Okamoto M, Craig PS.
  • 雑誌名: Southeast Asian Journal of Tropical Medicine and Public Health 37 (Suppl 3) ページ: 82-90
  • 査読あり
• [雑誌論文] Neurocysticercosis : clinical manifestation, neuroimaging, serology and molecular confirmation of histopathologic specimens (2006)
  • 著者名/発表者名: Ito A, Takayanagui OM, SakoY, Sato MO, Odashima NS, Yamasaki H, Nakaya K, Nakao M
  • 雑誌名: Southeast Asian Journal of Tropical Medicine and Public Health 37 (Suppl 3) ページ: 74-81
  • 査読あり
• [雑誌論文] Simultaneous alveolar and cystic echinococcosis of the liver (2006)
  • 著者名/発表者名: Yang YR, Liu XZ, Vuitton DA, Bartholomot B, Wang YH, Ito A, Craig PS, McManus DP
  • 雑誌名: Transactions of the Royal Society of Tropical Medicine and Hygiene 100 ページ: 597-600
  • 査読あり
• [雑誌論文] Evidence and potential for transmission of human and swine Taenia solium cysticercosis in the Piracuruca region, Piaui, Brazil (2006)
  • 著者名/発表者名: Sato MO, Cavalcante V, Sako Y, Nakao M, Yamasaki H, Yatsuda P, Nakaya K, Ito A
  • 雑誌名: American Journal of Tropical Medicine and Hygiene 75 ページ: 933-935
  • 査読あり
• [雑誌論文] A case of intramuscular cysticercosis diagnosed definitively by mitochondrial DNA analysis of extremely calcified cysts (2006)
  • 著者名/発表者名: Yamasaki H, Nagase T, Kiyoshige Y, Suzuki M, Nakaya K, Itoh Y, Sako Y, Nakao M, Ito A
  • 雑誌名: Parasitology International 55 ページ: 127-130
  • 査読あり
• [雑誌論文] Echinococcus multilocularis: developmental stage-specific expression of antigen B 8-kDa-subunits (2006)
  • 著者名/発表者名: Mamuti W, Sako Y, Xiao N, Nakaya K, Nakao M, Yamasaki H, Lightowlers MW,Craig PS, Ito A
  • 雑誌名: Experimental Parasitology 113 ページ: 75-82
  • 査読あり
• [雑誌論文] Recent advances in characterization of Echinococcus antigen B (2006)
  • 著者名/発表者名: Mamuti W, Sako Y, Nakao M, Xiao N, Nakaya K, Ishikawa Y, Yamasaki H, Lightowlers MS, Ito A
  • 雑誌名: Parasitology International 55 ページ: S57-S62
  • 査読あり
• [雑誌論文] Mitochondrial DNA diagnosis for taeniasis and cysticercosis (2006)
  • 著者名/発表者名: Yamasaki H, Nakao M, Sako Y, Nakaya K, Sato MO, Ito A
  • 雑誌名: Parasitology International 55 ページ: S81-S85
  • 査読あり
• [雑誌論文] Usefulness of severe combined immunodeficiency (scid) and inbred mice for studies of cysticercosis and echinococcosis (2006)
  • 著者名/発表者名: Nakaya K, Mamuti W, Xiao N, Sato MO, Wandra T, Nakao M, Sako Y, Yamasaki H, Ishikawa Y, Craig PS, Schantz PM, Ito A
  • 雑誌名: Parasitology International 55 ページ: S91-S97
  • 査読あり
• [雑誌論文] Taeniasis/cysticercosis in Papua (Irian Jaya), Indonesia (2006)
  • 著者名/発表者名: Margono SS, Wandra T, Swasono MF, Murni S, Craig PS, Ito A
  • 雑誌名: Parasitology International 55 ページ: S143-S148
  • 査読あり
• [雑誌論文] Genetic variation in Taenia solium (2006)
  • 著者名/発表者名: Campbell G, Garcia HH, Nakao M, Ito A, Craig PS.
  • 雑誌名: Parasitology International 55 ページ: S121-S126
  • 査読あり
• [雑誌論文] Recombinant antigens for serodiagnosis of cysticercosis and echinococcosis (2006)
  • 著者名/発表者名: Sako Y, Nakao M, Nakaya K, Yamasaki H, Ito A
  • 雑誌名: Parasitology International 55 ページ: S69-S73
  • 査読あり
• [雑誌論文] The Echinococcosis working group in China (2006)
  • 著者名/発表者名: Craig PS
  • 雑誌名: Parasitology International 55 ページ: S221-S225
  • 査読あり
• [雑誌論文] Echinococcus shiquicus, a new species from the Qinghai-Tibet plateau region of China: discovery and epidemiological implications (2006)
  • 著者名/発表者名: Xiao N, Qiu J, Nakao M, Li T, Yang W, Chen X, Schantz PM, Craig PS, Ito A
  • 雑誌名: Parasitology International 55 ページ: S233-S236
  • 査読あり
• [雑誌論文] High prevalence of Taenia saginata taeniasis and status of Taenia solium cysticercosis in Bali, Indonesia, 2002-2004 (2006)
  • 著者名/発表者名: Wandra T, Susisna P, Dharmawan NS, Margono SS, Sudewi R, Suroso T, CraigPS, Ito A
  • 雑誌名: Transactions of the Royal Society of Tropical Medicine and Hygiene 100 ページ: 346-353
  • 査読あり
• [雑誌論文] Conservation of the vaccine antigen gene, TSOL18, among genetically variant isolates of Taenia solium (2006)
  • 著者名/発表者名: Gauci CG, Ito A, Lightowlers MW
  • 雑誌名: Molecular and Biochemical Parasitology 146 ページ: 101-104
  • 査読あり
• [雑誌論文] Magnetic resonance imaging of alveolar echinococcosis experimentally induced in the rat lung (2006)
  • 著者名/発表者名: Asanuma T, Kawahara T, Inanami O, Nakao M, Nakaya K, Ito ATakiguchi M, Hashimoto A, Kuwabara M
  • 雑誌名: Journal of Veterinary Medical Science 68 ページ: 15-20
  • 査読あり
• [雑誌論文] Echinococcosis in Tibetan populations, western Sichuan province, China (2006)
  • 著者名/発表者名: Li T, Qiu J, Yang W, Craig PS, Chen X, Xiao N, Ito A, Giraudoux P, Mamuti W, Yu W, Schantz PM.
  • 雑誌名: Emerging Infectious Diseaess 11 ページ: 1866-1873
  • 査読あり
• [雑誌論文] evaluation of clinical and serological data from Taenia solium cysticercosis patients in eastern Inner Mongolia autonomous region, China (2006)
  • 著者名/発表者名: Ikejima T, Piao ZX, Sako Y, Sato MO, Bao S, Si R, Yu F, Zhang CL, Nakao M, Yamasaki H, Nakaya K, Kanazawa T, Ito A
  • 雑誌名: Transactions of the Royal Society of Tropical Medicine and Hygiene 99 ページ: 625-630
  • 査読あり
• [雑誌論文] Echinococcus muiltilocularis confirmed on Kunashiri island, 15 kilometers from the eastern part of Hokkaido, Japan (2006)
  • 著者名/発表者名: Sato M, Nakaya K, Nakao M, Xiao N, Yamasaki H, Sako Y, Naitoh Y, Kondo S, Kobayashi M, Ohtaishi N, Ito A
  • 雑誌名: American Journal of Tropical Medicine and Hygiene 72 ページ: 284-288
  • 査読あり
• [雑誌論文] Echinococcus shiquicus n. sp., a taeniid cestode from Tibetan fox and plateau pika in China (2006)
  • 著者名/発表者名: Xiao N, Qiu J, Nakao M, Li T, Yang W, Chen X, Schantz PM, Craig PS, Ito A
  • 雑誌名: International Journal for Parasitology 35 ページ: 693-701
  • 査読あり
• [雑誌論文] Usefulness of recombinant Em18-ELISA to evaluate efficacy of treatment inpatients with alveolar echinococcosis (2006)
  • 著者名/発表者名: Fujimoto Y, Ito A, Ishikawa Y, Inoue M, Suzuki Y, Ohhira M, Ohtake T, Kohgo Y
  • 雑誌名: Journal of Gastroenterology 40 ページ: 426-431
  • 査読あり
• [学会発表] Asia-Africa science platform project sponsored by the Japan Society for the Promotion of Science (JSPS) (2008)
  • 著者名/発表者名: Ito A
  • 学会等名: Joint International Tropical Medicine Meeting 2008
  • 発表場所: Bangkok, Thailand.
  • 年月日: 2008-10-13
• [学会発表] Key note speech on Echinococcosis : Serological detection of patients and animals (2008)
  • 著者名/発表者名: Ito A
  • 学会等名: 17th International Congress for Tropical Medicine and Malaria
  • 発表場所: Cheju, Korea.
  • 年月日: 2008-10-10
• [学会発表] Human echinococcosis on Eastan Tibetan plateau, China (2008)
  • 著者名/発表者名: Ito A.
  • 学会等名: 17th International Congress for Tropical Medicine and Malaria
  • 発表場所: Cheju, Korea.
  • 年月日: 2008-10-10
• [学会発表] Human echinococcosis in Qinghai, China (2008)
  • 著者名/発表者名: Ito A
  • 学会等名: 17th International Congress for Tropical Medicine and Malaria
  • 発表場所: Cheju, Korea.
  • 年月日: 2008-10-01
• [学会発表] Echinococcosis : Serodiagnosis for detection of patients and molecular identification of parasites (2008)
  • 著者名/発表者名: Ito A
  • 学会等名: 10th European Multi-colloquium of Parasitology
  • 発表場所: Paris, France
  • 年月日: 2008-08-25
• [学会発表] Molecular and serological diagnosis of taeniasis and cysticercosis in Asia and the Pacific (2007)
  • 著者名/発表者名: Ito A
  • 学会等名: Joint International Tropical Medicine Meeting 2007
  • 発表場所: Bangkok, Thailand
  • 年月日: 2007-11-30
• [学会発表] Key note speech on Developmental stage- specific gene expression in Echinococcus multilocularis and E. granulosus (2007)
  • 著者名/発表者名: Ito A
  • 学会等名: 22nd International Congress of Hydatidology
  • 発表場所: Athens, Greece
  • 年月日: 2007-05-16
• [学会発表] Keynote speech on CestodeBiology, ICOPA XI (2006)
  • 著者名/発表者名: Ito A
  • 学会等名: ICOPA XI
  • 発表場所: Glasgow, UK
  • 年月日: 20060806-11
• [学会発表] The present situation of taeniasis and cysticercosis in Asia and the Pacific (2006)
  • 著者名/発表者名: Ito A
  • 学会等名: Joint International Tropical Medicine Meeting 2006
  • 発表場所: Bangkok, Thailand.
  • 年月日: 2006-12-01
• [学会発表] Serological and molecular tools for diagnosis. Symposium on Clinical management of Alveolar Echinococcosis (2006)
  • 著者名/発表者名: Ito A
  • 学会等名: 55th Annual Meeting of American Society of Tropical Medicine and Hygiene
  • 発表場所: Atlanta, USA
  • 年月日: 2006-11-15
• [図書] The World Federation of Neurocysticercosis. Clinical Neurocysticercosis: Immunodiagnosis and molecular diagnosis for cysticercosis (2009)
  • 著者名/発表者名: Ito A, Takayanagui OM
  • 出版者: usefulness of cerebrospiral fluid (CSF) and serum samples(in press)
• [図書] Oxford University Press. Zoonoses : Taenia solium (2009)
  • 著者名/発表者名: Flisser A, Craig PS, Ito A
  • 出版者: Taenia saginata and Taenia asiatica (in press)
• [図書] CRC Press, Taylor & Francis Group. Handbook of Nucleic Acid Purification (ed. Liu D) Chapter13 (2009)
  • 著者名/発表者名: Okamoto M, Ito A
  • 総ページ数: 271-291
  • 出版者: Purification of Nucleic Acids from Helminthes
• [図書] CRC Press, Taylor & Francis Group. Food-born Pathogens (ed. Liu D) (2009)
  • 著者名/発表者名: Ito A, Nakao M, Sako Y, Nakaya K, Yanagida T, Okamoto M
  • 総ページ数: 827-838
  • 出版者: Chapter 62. Taeniasis
• [図書] Blackwell Publishing (2007)
  • 著者名/発表者名: Ito A, Baker DG
  • 総ページ数: 37-42
  • 出版者: Parasites of Laboratory Animals