| 研究課題/領域番号 |
17F17065
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| 研究機関 | 大阪大学 |
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研究代表者 |
松本 卓也 大阪大学, 理学研究科, 教授 (50229556)
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| 研究分担者 |
LIVSHITS GIDEON 大阪大学, 理学(系)研究科(研究院), 外国人特別研究員
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| 研究期間 (年度) |
2017-04-26 – 2019-03-31
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| キーワード | DNA electronics / field-assisteddepo. / nanofluidics / liquid metals |
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| 研究実績の概要 |
1. I set up for nanopipette system: For nanopipette control, implemented electronic circuit was designed and manufactured for current-detection of nanopipette proximity to surface. I Wrote Labview code to control motor stage for controllable nanopipette deposition.
2. I set up purchased Plan Fluor objective for fluorescence imaging.
3. I performed successful ligation and phenol-chloroform purification of high molecular weight Lambda-DNA, Successful staining of ligated DNA and deposition on APTES-coated Piranha-treated glass slides. Ligated Lambda-DNA was length ~100-200 um, similar to T4-DNA standard.
3. I prepared microelctrodes: I fabricated successfully mask and gate electro(gold on glass). Several attempts at deposition of DNA on glass. Large DNA fragments are visible inside nanopipette.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
1.Language barrier makes it difficult to fully integrate into the scientific discussions
2.Purchasing of equipment takes long time.
3.New promising results and new directions of research
4.Good feedback and high quality of research partners means that problems get solved quickly and efficiently.
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| 今後の研究の推進方策 |
1.Overcome problem with long-DNA moving through the nanopipette.
2.Release and deposition of DNA through nanopipette pore using syringe pump.
3.Assemble high-voltage (direct current) power supply for patterning of DNA on glass surface using back gate architecture.
4.Fabrication of smaller pore sizes, and extrusion of GaIn through these smaller cavities to obtain finer nanowires.
5.Finite-element simulation of GaIn wire properties and elastic response with the guidance of Eng. David Livshits.
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