研究実績の概要 |
This project investigated the role of neutrophil extracellular traps (NETs) during malaria infection. The major achievement of this project was to develop an accurate, high throughput method for the characterization of NETs. We achieved this through the development of a novel 3D cell culture method which we used to maintain a single cell solution of neutrophils and NETs. Cells were analyzed by imaging flow cytometry and six distinct cell types could be identified, including NETs, NET precursors, and non-NET dead cells. An automated analysis algorithm was development to quantify these cell types rapidly and in an unbiased manner. We applied this method to provide the first analysis of NETs in the circulation in mice infected with the murine malaria parasite Plasmodium yoelii.
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