| 研究課題/領域番号 |
17F17389
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| 研究機関 | 国立研究開発法人理化学研究所 |
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研究代表者 |
城口 克之 国立研究開発法人理化学研究所, 生命システム研究センター, ユニットリーダー (00454059)
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| 研究分担者 |
JIN JIANSHI 国立研究開発法人理化学研究所, 生命システム研究センター, 外国人特別研究員
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| 研究期間 (年度) |
2017-10-13 – 2020-03-31
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| キーワード | T cell / cell-cell interaction / microscopy |
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| 研究実績の概要 |
We have been developing a technique to understand cell-cell interaction of the T cell system. In this fiscal year, we have cultured T cells which express a specific T cell receptor, and also a cell which is supposed to interact with the T cell. To observe the interaction of these cells, we have developed a microscopy platform which is able to scan interested cells. Using this platform, we have successfully observed cell-cell interaction. In addition, we have found several different cases of the interactions by scanning. We also tried to make the scanning system high-throughput. For this aim, we have investigated the scanning rate of our current microscope system, and tried to improve the rate.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
(1) Developing a platform for scanning the cell interaction.
Using imaging, we successfully found an interested interaction of T cell and target cell. In addition, we have found several different cases of their interactions. On the other hand, for the high-throughput scanning, we have checked the scanning rate of the current microscope system, and tried to improve it.
(2) Making the constructs and express different antigens on the target cells.
We have cultured the T cells which express a specific T cell receptor, and the target cells which present a specific antigen for that T cell, and also several non-specific target cells which present non-specific antigens for that T cell.
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| 今後の研究の推進方策 |
In this fiscal year, we will do cell picking-up, sequencing, and data analysis.
(1) Picking up an interested cell: an interested cell will be scanned using a microscopy system, and a recognized cell will be picked up to a separate tube.
(2) Amplification of a target gene of a picked up cell: an mRNA of gene will be reverse transcribed to cDNA after the cell is lysed in each tube. Then, the cDNA will be amplified by PCR.
(3) Sequencing: amplified product will be sequenced.
(4) Data analysis: after sequencing, I will do data analysis by developing a pipeline to identify the sequences.
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