| 研究課題/領域番号 |
17J00362
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| 研究機関 | 沖縄科学技術大学院大学 |
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研究代表者 |
TSAI Hsieh Fu 沖縄科学技術大学院大学, 科学技術研究科, 特別研究員(DC1)
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| 研究期間 (年度) |
2017-04-26 – 2020-03-31
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| キーワード | neuron culture / deep learning / label-free segmentation / electrotaxis / temperature controller |
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| 研究実績の概要 |
1. A facile PDMS treatment protocol is developed to support the growth of the most fastidious cell type in microsystems. 2. A open source versatile platform is developed for lab on chip experiments. It is very easy to program and can connect with various sensors and outputs. 3. A modified deep learning platform DeepCell is used for label-free cell segmentation and tracking the glioma cell migration. 4. Influence of extracellular matrix for glioma cell adhesion and electrotaxis. I've started to analyze the influence of ECM proteins on glioma cell adhesion and electrotaxis.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
I am proceeding as research as planned.
A microfluidic chip is designed and used for collecting of glioma cell electrotaxis under various microenvironment conditions. An deep-learning based label free phase contrast cell segmentation platform is also developed for resolving a major data analysis bottleneck for cell migration studies. An opensource temperature controller is also developed to fulfill partially the automated chip platform. Based on the advances in the first year, for the next year, i can move on to collect more cell electrotaxis data and investigate further how microenvironment affects the electrotaxis.
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| 今後の研究の推進方策 |
The primary work for next year is to explore more on the electrotaxis of glioma cells when there are various coexisting chemical ligands in the microenvironment.
Secondly, I have tested building a microfluidic chip that creates orthogonal chemical and voltage gradients. However, the stability of the gradients is prone to system perturbance (i.g. syringe pump instability, chip fabrication inperfections). Redesigning a microfluidic chip with stable gradient generation will be a key step in next year.
Thirdly, the effective charge of native proteins is a grossly overlooked characteristic that is very important in our configuration. I intend to examine the effective charge of proteins by capillary electrophoresis examination this year.
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