| 研究実績の概要 |
A. A two-layer hybrid microfluidic device is developed for high throughput electrotaxis study. A robust hybrid microfluidic device composed of PMMA/PDMS is developed with 2-layer design for high throughput electrotaxis study of glioblastoma. A method to uniformly seed sparse cells in microchannel is also achieved by balancing inlet/outlet pressure.
B. Electrotaxis of glioblastoma requires optimal laminin-containing extracellular matrix. Using the platform and the label-free cell tracking software we developed before, T98G and U-251MG glioblastoma electrotaxis are investigated and been found to depend on laminin extracellular matrix
C. Electrotaxis of glioblastoma cell models can be heterogenous. We have also found that T98G and U-251MG despite being molecularly and phenotypically typed similarly as mesenchymal glioblastoma, the electrotactic behavior is completely different. The result may reflect the heterogeneity of the brain cancer.
D. Electrotaxis of glioblastoma cells are mediated by voltage-gated ion channels. The calcium signaling plays a pivotal role in cell electrotaxis as well as various aspects of glioblastoma biology such as drug resistance and proliferation. We investigated if the voltage-gated calcium channels (VGCC) mediate the electrotaxis of glioblastoma and that different VGCCs are involved in different glioblastoma cell lines. Other ion channels such as voltage-gated potassium channels and the amiloride-sensitive sodium channels also mediates the glioblastoma cell electrotaxis. showing the importance of confirming patient-specific ion expression profile.
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