| 研究実績の概要 |
Designed a cell culturing system for human cell lines, purchased equipment, and built a system that allows for aseptic handling and storage of cells, cell culturing, and exposure of cells to hazardous chemicals. Developed methods for the extraction of DNA from human cells, digestion of DNA and preparation for LC/ESI-MS/MS analyses. Methods for LC/ESI-MS/MS analyses were developed. DNA adductomics analyses of Hep G2 cells were conducted after exposure to the dietary-related, monoaromatic chemical, safrole. Comparative analyses through the construction of DNA adductome maps revealed safrole-derived DNA adducts and preliminary identification of such adducts was conducted by LC/ESI-MS/MS product ion scan fragmentation analyses. Results of the first years' work were presented at two national meetings.
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