Establishment of DNA adductomics to study human cells

2019 年度 実績報告書

• 研究課題/領域番号: 17K00555
• 研究機関: 横浜市立大学
• 研究代表者: Robert Kanaly 横浜市立大学, 理学部, 教授 (00448656)
• 研究期間 (年度): 2017-04-01 – 2020-03-31
• キーワード: Adductomics

研究実績の概要

Nucleic acid adductomics analyses utilizing human cell lines were continued for DNA adductomics and DNA/RNA adductomics. High resolution-(HR-)MS methodologies were successfully applied in conjunction with traditional adductomics approaches. Exposure assays that utilized the model 2'-deoxynucleoside, 2'-deoxyguanosine were conducted using 1,2-naphthoquinone and arylhydroxylamines and analyzed by LC/ESI-MS/MS, LC/ESI-HR/MS and NMR. Syntheses, purification and confirmation of arylhydroxylamine adducts were conducted.

研究成果

• [雑誌論文] Characterization of N-(2,6-dimethylphenyl)hydroxylamine adducts of 2′-deoxyguanosine under weakly basic conditions (2020)
  • 著者名/発表者名: Matsui, T., N. Yamada, H. Kuno and R.A. Kanaly
  • 雑誌名: Chemosphere 巻: 252 ページ: 126530
  • DOI: 10.1016/j.chemosphere.2020.126530
  • 査読あり / 国際共著
• [雑誌論文] Chemical and genomic analyses of polycyclic aromatic hydrocarbon biodegradation in Sphingobium barthaii KK22 reveals divergent pathways in soil sphingomonads (2020)
  • 著者名/発表者名: Maeda, A.H., S. Nishi, Y. Hatada, Y. Ohta, K. Misaka, M. Kunihiro, J.F. Mori and R.A. Kanaly
  • 雑誌名: International Biodeterioration & Biodegradation 巻: 151 ページ: 104993
  • DOI: 10.1016/j.ibiod.2020.104993
  • 査読あり / 国際共著
• [雑誌論文] Formation of bulky DNA adducts by non-enzymatic production of 1,2-naphthoquinone-epoxide from 1,2-naphthoquinone under physiological conditions (2019)
  • 著者名/発表者名: Matsui, T., N. Yamada, H. Kuno and R.A. Kanaly
  • 雑誌名: Chemical Research in Toxicology 巻: 32 ページ: 1760-1771
  • DOI: 10.1021/acs.chemrestox.9b00088
  • 査読あり / 国際共著
• [雑誌論文] In vitro DNA/RNA adductomics to confirm DNA damage caused by benzo[a]pyrene in the Hep G2 cell line. (2019)
  • 著者名/発表者名: Takeshita, T., and R.A. Kanaly
  • 雑誌名: Frontiers in Chemistry 巻: 7 ページ: 491
  • DOI: 10.3389/fchem.2019.00491
  • 査読あり / オープンアクセス / 国際共著
• [雑誌論文] Triple quadrupole mass spectrometry comparative DNA adductomics of Hep G2 cells following exposure to safrole (2019)
  • 著者名/発表者名: Takeshita, T., F. Tao, N. Kojima and R.A. Kanaly
  • 雑誌名: Toxicology Letters 巻: 300 ページ: 92-104
  • DOI: 10.1016/j.toxlet.2018.10.023
  • 査読あり / 国際共著
• [学会発表] Investigation of an adduct formation mechanism for N-oxidized aniline derivatives with 2’-deoxyguanosine (2019)
  • 著者名/発表者名: Matsui, T., N. Yamada, H. Kuno and R.A. Kanal
  • 学会等名: The 37th Medicinal Chemistry Symposium (MSC2019)
• [学会発表] Verification of DNA adduct formation derived from chemical compounds by DNA/RNA adductome methods and in silico: ab initio calculation analyses (2019)
  • 著者名/発表者名: Takeshita, T., H. Sakagami, M. Tachikawa and R.A. Kanaly
  • 学会等名: Joint Meeting of the 6th Asian Congress on Environmental Mutagens (ACEM) and the 48th Annual Meeting of the Japanese Environmental Mutagen Society (JEMS)
  • 国際学会
• [学会発表] Examination of the micronucleus test in T24 human bladder carcinoma cells (2019)
  • 著者名/発表者名: Yamazeki, Y., R. Kanauchi, A. Nagano, R.A. Kanaly and T. Takeshita
  • 学会等名: Joint Meeting of the 6th Asian Congress on Environmental Mutagens (ACEM) and the 48th Annual Meeting of the Japanese Environmental Mutagen Society (JEMS)
  • 国際学会
• [学会発表] SO_1646 encodes a diguanylate cyclase that determines intracellular cyclic di-GMP levels and regulates biofilm formation and current generation by Shewenella oneidensis MR-1 (2019)
  • 著者名/発表者名: Matsumoto, A., R. Koga, R.A. Kanaly, A. Kouzuma and K. Watanabe
  • 学会等名: International Society for Microbial Electrochemistry and Technology 7 (ISMET 7 2019)
  • 国際学会
• [学会発表] The oil-eating gangs: metagenomics revealed the multi-species oil hydrocarbon-degrading soil bacterial consortium evolved in the lab-scale enrichment (2019)
  • 著者名/発表者名: Mori, J.F., Y. Tomiyama and R.A. Kanaly
  • 学会等名: The 33rd Annual Meeting of the Japanese Society of Microbial Ecology (JSME)
  • 国際学会
• [学会発表] Investigation of a hydrocarbon pollutant-degrading bacterial consortium and Bradyrhizobium sp. strain KK5 (2019)
  • 著者名/発表者名: Tomiyama, Y., J.F. Mori and R.A. Kanaly
  • 学会等名: The 33rd Annual Meeting of the Japanese Society of Microbial Ecology (JSME)
  • 国際学会