多能性幹細胞由来心筋永久機関：循環進行波に基づく加速心筋細胞成熟法の開発

2017 年度 実施状況報告書

• 研究課題/領域番号: 17K14624
• 研究機関: 大阪大学
• 研究代表者: 李 俊君 大阪大学, 医学系研究科, 特任研究員(常勤) (10723786)
• 研究期間 (年度): 2017-04-01 – 2019-03-31
• キーワード: pluripotent stem cells / cardiomyocytes / maturation

研究実績の概要

In the last year, our projects progressed smoothly. We have design and optimized the self-organized tissue ring technology for promoting high-speed cardiomyocytes beating. This technique could be used for rapid and controllable cardiac maturation without any external stimulation.The achievements are specifically:
1.Different parameters have been discussed for controlling the beating frequency of cardiomyocytes. Up to 90% percent success rate could be achieved in the multi-well plate. 2.The Traveling wave could work in several frequency mode and beat for more than 3 months. 3.A computational model has been created by using Matlab. The model could interpret the mechanism how the traveling waves are generated and maintained.

現在までの達成度 (区分)

2: おおむね順調に進展している

理由

So far, we have almost achieved all the planned step for first year. We are optimistic that the project could progress smoothly in the following year.

今後の研究の推進方策

Based on previous work, we will further characterize the maturation and performance of cardiomyocytes in self-organized tissue ring after training. The Real-time PCR, immunostaining, transmission electron microscope,contractile force generation and other methods will be used to characterize the maturation of cardiomyocytes. The data will be compared with the other reports.
2. Large-scale production method for matured tissue ring will also be established. Rings made of ~10e7 cardiomyocytes will be made within one plate.The design of plating well will be optimized to improve the robustness of traveling wave. The interruption of accidental conditions will be minimized by improving the well structure.

研究成果

• [学会発表] Generating functional anisotropic and 3D tissue-like construct using induced pluripotent stem cell-derived cardiomyocytes. (2017)
  • 著者名/発表者名: Li Junjun
  • 学会等名: Biomedical Engineering Society Annual Meeting
  • 国際学会