| 研究実績の概要 |
We employed various multi-omics approaches to determine clonality structures of HTLV-1 infected individuals using three different criteria including the provirus integration sites, T cell receptor rearrangements and somatic mutations. ① Clonality analysis based on integration sites using NGS technology suggested its possible applications in molecular diagnosis,and predicting prognosis (Blood advances 2017,Human genomics 2017).We also developed a new and rapid method for reliable clonality analysis using MinION Nanopore sequencer.② We showed ATL displays a high degree of ITH and a complex subclonal structure based on mutation(Neoplasia 2018)③ We found that TCR clonality profiling by RNA sequencing is useful for prognostic purposes and personalizing ATL diagnosis (npj genom. Med. 2019).
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