| 研究課題/領域番号 |
17K15053
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| 研究機関 | 総合研究大学院大学 |
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研究代表者 |
QUINTIN LAU 総合研究大学院大学, 先導科学研究科, 特別研究員 (60794518)
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| 研究期間 (年度) |
2017-04-01 – 2019-03-31
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| キーワード | transcriptome / anuran / frog / chytridiomycosis |
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| 研究実績の概要 |
-Completed study of transcriptome and MHC class II characterization of three Ranidae species, now published in BMC Genomics.
-Conducted and completed study characterizing TLR2 and TLR4 genes in the three Ranidae species, manuscript is now in review.
-Studied expression levels of MHC class I and II in tadpoles during development.
-Collected RNA samples from nine other Japanese frog species. From RNA, generated cDNA libraries for Illumina sequencing, conducted assembly and blast search. Collated MHC sequences from the nine species.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
3: やや遅れている
理由
During the first half of FY2017, I was continuing and completing related research in three Ranidae species. This research is directly related to the currently-funded research. For example, I completed a transcriptome study of the three Ranidae species, and characterized MHC class II genes (including analyses of sequences and linking it to resistance to chytridiomycosis). These approaches will be repeated and applied to nine other Japanese frog species in the currently-funded research project.
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| 今後の研究の推進方策 |
-Analyze MHC class I and II sequences from the newly acquired transcriptome data of nine Japanese frog species. This will include supertype analyses, to compare MHC sequences across disease resistant and susceptible species across the world. We will also begin analyses to test predicted binding of chytrid-related peptides by MHC receptors.
-With assistance of Australian collaborators listed in the application, we will collect RNA from two Australian species of frogs, one that is susceptible and one that is resistant. We will then construct cDNA libraries for Illumina sequencing, and compare sequences of MHC and other immune genes with Japanese frogs.
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| 次年度使用額が生じた理由 |
-Costs for field sampling as well as consumables for sample preparation and genotyping were lower than expected. This was partly because we purchased several consumables using remaining funds prior to this project.
-For the next fiscal year we plan to purchase new kits for transcriptome library, and conduct Illumina sequencing of additional samples from Australian frogs species. I will also attend an International conference to present our results. In addition, we plan to purchase more consumables, and have local research meetings, and purchase a computer to assist in computational analyses.
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