• 研究課題をさがす
  • 研究者をさがす
  • KAKENの使い方
  1. 課題ページに戻る

2018 年度 実施状況報告書

Saving the frog: Understanding how Japanese frogs are resistant to a deadly worldwide fungal disease through in silico and in vitro assays of MHC and related immune genes

研究課題

研究課題/領域番号 17K15053
研究機関総合研究大学院大学

研究代表者

QUINTIN LAU  総合研究大学院大学, 先導科学研究科, 特別研究員 (60794518)

研究期間 (年度) 2017-04-01 – 2020-03-31
キーワードRana / MHC / chytrid / frog
研究実績の概要

Study A- Expression of MHC class I and II in tadpoles during development: Completed qPCR experiments to measure MHC expression in Rana ornativentris tadpoles, and also conducted normal PCR to detect presence of MHC amplification from Xenopus tadpoles. Prepared and submitted manuscript.

Study B-Transcriptome of numerous Japanese frog species to study immune genes: isolated MHC class I and II sequences and validated using cloning/sequencing. Completed phylogenetic and supertyping analyses of the MHC sequences. Found that all Japanese frogs share some common physiochemical properties in MHC II based on supertyping anaylsis. Conducted preliminary computer-based prediction of binding affinity between frog MHC sequence and chytrid fungus sequence. Other immune genes were also isolated from transcriptome data including innate immune genes (Toll-like receptors and antimicrobial peptides).

現在までの達成度 (区分)
現在までの達成度 (区分)

3: やや遅れている

理由

Study A: During the end of FY2018, we received heavy reviewer criticism related to our study of MHC expression in tadpoles, which drove us to the decision to continue this project with more robust and in-depth approach (i.e. transcriptome sequencing).

Study B: The isolation of MHC sequences from transcriptome data was complex. Since MHC is the most polymorphic gene in the genome, it was challenging to validate allele sequences based on transcriptome data alone (because each individual may have multiple MHC loci due to gene duplication). Thus, I had to conduct cloning and sequencing to confirm presence of true MHC alleles. This additional experimental work caused delay of subsequent analyses.

今後の研究の推進方策

Study A- conduct cDNA library sequencing of Rana and Xenopus tadpole samples, isolate differentially expressed genes (DEGs) as well as expression levels of MHC class I and II. Update the former manuscript for submission

Study B- complete MHC supertyping analyses. Conduct further analyses of predicting MHC binding, utilizing more diverse sequences from the chytrid fungus after consulting with Australian collaborators.

次年度使用額が生じた理由

Due to time constraints and delays in experimental work, the project has taken longer than expected to finish

  • 研究成果

    (4件)

すべて 2018 その他

すべて 国際共同研究 (1件) 雑誌論文 (2件) (うち国際共著 2件、 査読あり 2件、 オープンアクセス 1件) 学会発表 (1件)

  • [国際共同研究] James Cook University(オーストラリア)

    • 国名
      オーストラリア
    • 外国機関名
      James Cook University
  • [雑誌論文] Selective constraint acting on TLR2 and TLR4 genes of Japanese Rana frogs2018

    • 著者名/発表者名
      Quintin Lau, Takeshi Igawa, Tiffany A. Kosch and Yoko Satta
    • 雑誌名

      PeerJ

      巻: 6 ページ: e4842

    • DOI

      10.7717/peerj.4842

    • 査読あり / オープンアクセス / 国際共著
  • [雑誌論文] Genetic potential for disease resistance in critically endangered amphibians decimated by chytridiomycosis2018

    • 著者名/発表者名
      T. A. Kosch, C. N. S. Silva, L. A. Brannelly, A. A. Roberts, Q. Lau, G. Marantelli , L. Berger, L. F. Skerratt
    • 雑誌名

      Animal Conservation

      巻: - ページ: -

    • DOI

      10.1111/acv.12459

    • 査読あり / 国際共著
  • [学会発表] Understanding how Japanese frogs are resistant to a deadly worldwide fungal disease through in silico analyses of major histocompatibility complex (MHC)2018

    • 著者名/発表者名
      LAU, Quintin, IGAWA, Takeshi, KOSCH, Tiffany A. , and SATTA, Yoko
    • 学会等名
      SMBE 2018

URL: 

公開日: 2019-12-27  

サービス概要 検索マニュアル よくある質問 お知らせ 利用規程 科研費による研究の帰属

Powered by NII kakenhi