| 研究課題/領域番号 |
17K16994
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| 研究機関 | 国立研究開発法人理化学研究所 |
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研究代表者 |
Tu HungYa 国立研究開発法人理化学研究所, 多細胞システム形成研究センター, 訪問研究員 (10780835)
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| 研究期間 (年度) |
2017-04-01 – 2020-03-31
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| キーワード | retinal transplantation / rod bipolar cell / AII amacrine cell |
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| 研究実績の概要 |
The present study is aimed to figure out the causality between the inner retinal hyperactivity and the inactivation of upstream bipolar cell type commonly seen in retinal degeneration models. Re-activation of bipolar cells potentially resulted from suppression of the inner retinal hyperactivity is thus expected to improve the host retinal cell conditions and environments during transplantation therapy.
In contrast to the proposed work plan, the optimization of drug application for hyperactivity suppression has been conducted first in 2017, along with the setup of MEA recording/analysis and mouse iPSC-retina transplantation. In the meanwhile, the system has also been utilized for testing graft-host connection in other preparations including the xeno-transplantation case published in 2018.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
The primary MEA recording method to compare the transplantation efficacy in host retinas with or without inner retinal hyperactivity has been established with qualitative and quantitative analyses. By the MEA recording, the suppression of such hyperactivity may be cooperated with preliminary results from other ongoing experiments probing the effects of host aging condition, graft composition and ambient light environment, which will even more expand the proposed aims to cover the customization and optimization of retinal transplantation therapy in future clinical treatment.
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| 今後の研究の推進方策 |
Along with the systematic comparison of retinal transplantation efficacy in different host/graft conditions, the reasons of bipolar cell inactivation and inner retinal hyperactivity are required to be specified. The therefore required mouse resource for targeted patch clamp recording has been established and secured, and candidate ion channels important for manipulating the bipolar cell state have been suggested in literature reviewing and preliminary observation. Moreover, another inhibitory cell type is now being considered as a potential target for suppressing inner retinal hyperactivity/bipolar cell inactivation.
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| 次年度使用額が生じた理由 |
The travel expense has been lower than expected, partially because of the generous coverage by travel grant provided by the international conference attended. However, the cost will be raised along with the enrichment of data acquirement to cover the increased participation of both domestic and international conferences expected in the following year. Also, the expected expense for conducting targeted patch clamp recording is postponed to be utilized in the following 2 years.
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