| 研究課題/領域番号 |
17K17093
|
|---|
| 研究機関 | 九州大学 |
|---|
研究代表者 |
倪 軍軍 九州大学, 歯学研究院, 助教 (00783953)
|
|---|
| 研究期間 (年度) |
2017-04-01 – 2019-03-31
|
| キーワード | Alzheimer's disease / Microglia / Cathepsin E |
|---|
| 研究実績の概要 |
1, The CatE overexpressed microgla has been established based on the pEGFP-N3 plasmid. The overexpression efficiency has been checked by both RT-PCR and Western blotting.
2, The clearance activity of CatE has been checked by using CatE overexpressed microglia. The phagocytotic activity of microglia were not changed between WT and CatE overexpressed microglia, however, the digestion efficiency of uptaked Abeta were significantly increased in CatE overexpressed microglia.
3, The APP NL-G-F/NL-G-F/CatE-/- mice were picked up and maintained for future experiments.
|
| 現在までの達成度 (区分) |
現在までの達成度 (区分)
3: やや遅れている
理由
1, The CatE knock-out microglia were not obtained using Crispr-cas9 system using CatE target sequence inserted pCas-Guide, because of the low efficiency of transfection.
|
| 今後の研究の推進方策 |
1, Using the APP NL-G-F/CatE-/- mice brain samples to checked the Abeta accumulation in mice cortex and hippocampus.
2, Memory tests of APP NL-G-F/CatE-/- mice using Y-maze test, novel object recognition test and step-through passive avoidance test, when the mice number is enough.
|
| 次年度使用額が生じた理由 |
The incurring amount will be used for mouse behavior test and in-vivo experiments.
|
