| 研究実績の概要 |
Wild-type and Ywhag-/-, Ywhae-/- samples were collected at E15.5 and E18.5. We stained whole skeletons with alcian blue and alizarin red to identify cartilage and calcified tissues, respectively. Ywhag-/- showed similar level compared with wild-type, while Ywhae-/- embryos showed mild dwarfism, size of Ywhae-/- skeletons was smaller than wild-type.
Due to embryonic lethality of Ywhae-/- mice, wild-type and Ywhag-/-, Ywhae+/- samples were obtained from the mice at 4 weeks and 8 weeks of age. Body weight and bone volume of Ywhag-/- mice and Ywhae+/- mice were lower than that of wild-type mice, especially at 4-6 weeks of age.
The survival rate of Ywhag-/- mice at 8 weeks of age was lower than Ywhag+/+ and Ywhag+/- mice. Survival rate of Ywhae+/- mice was lower than that of wild-type.
|
