| 研究課題/領域番号 |
17K17139
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| 研究機関 | 北海道医療大学 |
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研究代表者 |
唐 佳 北海道医療大学, 歯学部, 助教 (80755992)
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| 研究期間 (年度) |
2017-04-01 – 2019-03-31
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| キーワード | Nephronectin / dentin regeneration / iMatrix411 / iMatrix511 |
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| 研究実績の概要 |
The purpose of this study was to investigate the effects of nephronectin (Npnt) in human dental pulp stem cells (hDPSCs). The results found Npnt mediates hDPSCs adhesion and spreading partially via RGD motif. Npnt enhanced the mRNA expression of ITGA1, ITGA4, ITGA7 and ITGB1 on day five. Npnt downregulated DSPP but significantly upregulated BSP at day 28. Moreover, Npnt and FCOL1 accelerated the matrix mineralization in hDPSCs. The findings implicated Npnt would be favorable to recruit hDPSCs and conducive to mineralization in hDPSCs. The combination of Npnt with hDPSCs may offer a promising approach for hard tissue regeneration.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
The hard tissue forming capacity of Npnt in hDPSCs has already been demonstrated by our research results.
It is thus believed our research is progressing smoothly.
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| 今後の研究の推進方策 |
In the future work, we intend to synthesize several artificial RGD-containing peptides derived from Npnt amino acid sequence and compare the hard tissue inducing capacities among them, in an effort to determine the most promising one to be applied in the in vivo animal study.
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| 次年度使用額が生じた理由 |
It is hard to use exactly the same amount that was allocated in the first fiscal year, the PI plans to use the remaining 3,086 together with the amount allocated for the next fiscal year (2018-2019) for procurement of experiment chemicals.
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