| キーワード | PIGA, / epilepsy, / E/I balance, / hippocampus, / thalamus, / ataxia, / learning and memory, / synapse |
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| 研究実績の概要 |
By using conditional knockout system, we successfully obtained mice with the ablation of PIGA specifically in inhibitory neurons(IKO), in forebrain excitatory neurons(EKO) and in thalamus neurons(TKO).The behavioral, EEG, histology and electrophysiology analysis showed that IKO and EKO but not TKO mice performed poorly in learning and memory task. IKO and EKO also showed obvious ataxia. It is notable that the susceptibility to kainic acid induced seizures was significantly increased in all three types of mutants. The synaptic density was changed in specific hippocampal subregions of all the mutants. There was a significant E/I balance change in IKO and EKO mice. In addition, AAV mediated PIGA expression in hippocampus could significantly suppress Kainic induced seizure in IKO and EKO.
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