| 研究課題/領域番号 |
18F18090
|
|---|
| 研究機関 | 京都大学 |
|---|
研究代表者 |
上高原 浩 京都大学, 農学研究科, 教授 (10293911)
|
|---|
| 研究分担者 |
DHAR PRODYUT 京都大学, (連合)農学研究科(研究院), 外国人特別研究員
|
|---|
| 研究期間 (年度) |
2018-10-12 – 2021-03-31
|
| キーワード | Cellulose nanocrystals / Nanomotors / Microfluidics / Biomedical |
|---|
| 研究実績の概要 |
The microfluidic device for the continuous production of Cellulose Nanocrystals (CNCs) was designed and is in process of fabrication through a series of sequential steps of photo-lithography. The fabricated microfluidic device will be used for optimization of production of CNCs using different acid systems, biomass source and enzymes. The system will be connected to an already available in-house continuous freeze drying unit, to produce CNCs in dried powdered form in a continuous fashion at high solid content. Simultaneously, in the second part of the study, strategic end-functionalization of CNCs are carried out using enzymes for fabrication of CNC based nanomotors which have potential biomedical applications. In present work, CNCs have been fabricated from two resources, Avicel and Micro crystalline cellulose and characterized for its morphology through electron microscopy studies. Thereafter, the CNCs were chemically modified with amine functionalities at the reducing end and the enzymes were immobilized. Currently, the end functionalization is confirmed through NMR studies using ionic liquid as solvent following a newer protocol. Thereafter, the study will be concluded by determining the enzyme stability and predicting the motion of the nanomotors under various peroxide environments. We have also developed a new process of fabricating scaffolds form wood-based resources using a very simple and green approach which have tremendous potential in biomedical area.
|
| 現在までの達成度 (区分) |
現在までの達成度 (区分)
3: やや遅れている
理由
Little bit slower than estimated, because of the time taken to arrange the facilities.
|
| 今後の研究の推進方策 |
A series of microfluidic reactors are currently developed with different designs through photo lithography methods. They will be tested and optimized for cellulose nanocrystal (CNC) production and understand the mechanism for its fabrication using different acids for hydrolysis (varying concentration and flow rate) and cellulase enzymes with possible provision to recycle the acid/ enzymes for several recycles. After CNC production, separation of CNCs on basis of charges will be done to remove acids from suspension and decrees it’s pH to neutral conditions. After that suspension will be feed to continuous spray drying equipment where the CNC suspension will be dried in form of powdered and the production rate per unit time will be calculated. CNCs produced with be characterized for the morphology, crystallinity, thermal properteis and other related structural and physico-chemical properties.
In the second part of the project work, we are developing end-functionalized CNCs modifying it with enzymes which have high peroxidase activity. The enzyme modified CNCs will be characterized to determine their stability, enzyme activity and conformational changes in structure. In presence of peroxidase the motion of the nanomotors will be tracked and the average velocities under different concentration, pH and ionic strength of fuel will be determined. CNCs are known for their sustainability, biocompatibility and non-toxicity and therefore, the developed nanomotors will be under simulated body fluids for potential biomedical applications.
|
