| 研究課題/領域番号 |
18F18101
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| 研究機関 | 横浜市立大学 |
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研究代表者 |
谷口 英樹 横浜市立大学, 医学研究科, 教授 (70292555)
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| 研究分担者 |
NIE YUNZHONG 横浜市立大学, 医学部, 外国人特別研究員
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| 研究期間 (年度) |
2018-04-25 – 2020-03-31
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| キーワード | hiPSC / Organoid / Hepatocyte / Hematopoietic cells / Cell-cell interactions / Liver disease / Liver organogenesis / CD14 |
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| 研究実績の概要 |
In this study, we are going to understand how the dynamic interactions between hematopoietic and hepatic cells regulate liver development, and to generate the functional liver organoid that can be used for liver disease study and model. For this purpose, we try to establish an organoid to recapitulate hematopoietic-hepatic interactions during early liver organogenesis. In the last year, we successfully established a hematopoietic-hepatic organoid, and found that hematopoietic cells could improve hepatic maturation and hepatic microenvironment helped to maintain the viability of hematopoietic cells. Moreover, we identified that CD14+ cells were that effective hematopoietic population, and noticed that hepatic lineages derived signals could promote the differentiation of CD14+ cells.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
With the established hematopoietic-hepatic organoid, we recapitulated the hematopoietic-hepatic interaction during organoid development, and we figured out the effective hematopoietic population that can promote hepatocyte maturation. These findings confirmed the value of this organoid system in further revealing the dynamic interactions between hematopoietic-hepatic cells in human being. Therefore, this plan is expected to progress smoothly.
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| 今後の研究の推進方策 |
In this year, we are going to further explore the underlying mechanisms of the dynamic interactions by comprehensively analyzing hepatic cells and hematopoietic cells with RNA sequencing and bioinformatics analysis, and try to in vivo verify symbiotic relationship between liver organogenesis and hematopoiesis with animal models. Moreover, we also want to characterize the function of hematopoietic-liver organoid in liver injury mice. Finally, we will summarize the results and publish related papers.
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