| 研究課題/領域番号 |
18F18384
|
|---|
| 研究機関 | 国立研究開発法人国立精神・神経医療研究センター |
|---|
研究代表者 |
株田 智弘 国立研究開発法人国立精神・神経医療研究センター, 神経研究所 疾病研究第四部, 室長 (70535765)
|
|---|
| 研究分担者 |
CONTU VIORICA 国立研究開発法人国立精神・神経医療研究センター, 神経研究所 疾病研究第四部, 外国人特別研究員
|
|---|
| 研究期間 (年度) |
2018-11-09 – 2021-03-31
|
| キーワード | lysosomal degradation / RNautophagy / lysosome |
|---|
| 研究実績の概要 |
RNautophagy/DNautophagy (RDA) is a novel RNA/DNA degradation process performed by lysosomes, during which RNA/DNA is directly imported into lysosomes in the presence of ATP and then degraded. RNautophagy was found to degrade RNA linked to the pathology of neurodegenerative diseases such as amyotrophic lateral sclerosis. In addition, we have reported that RNautophagy is an important pathway for intracellular RNA degradation and that SIDT2 mediates the translocation of RNA/DNA across the lysosomal membrane during RDA. Through this study, the fellow aims to characterize the molecular machinery of RDA and investigate the physiological significance and possible implications of RNautophagy in neurodegeneration. New insights into the molecular machinery that intermediates the direct uptake of nucleic acids by lysosomes are being provided. The fellow elucidated whether nucleic acids are taken up by lysosomes through a transporter or through microautophagy during RDA.
|
| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
Experiments are on schedule. New insights into the molecular machinery that intermediates the direct uptake of nucleic acids by lysosomes are being provided. The fellow elucidated whether nucleic acids are taken up by lysosomes through a transporter or through microautophagy during RDA.
|
| 今後の研究の推進方策 |
The fellow intends to elucidate whether SIDT2 is a channel protein and whether oligonucleotides can pass through the pore formed by SIDT2. An electrophysiological method, the droplet contact method, and electron microscopy will mainly be used.
|
